Evaluation of the cytotoxic and cytostatic activities of alkaloid extracts from different parts of Peganum harmala L.(Zygophyllaceae)

Bournine, L; Bensalem, Sihem; Fatmi, S.; Bedjou, F; Mathieu, Véronique; Iguer-Ouada, M.; Kiss, R; Duez, Pierre

doi:10.1016/j.eujim.2016.10.002

Article (Scientific journals)

Evaluation of the cytotoxic and cytostatic activities of alkaloid extracts from different parts of Peganum harmala L.(Zygophyllaceae)

Bournine, L; Bensalem, Sihem; Fatmi, S. et al.

2017 • In European Journal of Integrative Medicine, 9, p. 91-96

Peer reviewed

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https://hdl.handle.net/20.500.12907/9900

DOI
10.1016/j.eujim.2016.10.002

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Abstract :

[en] Introduction: Peganum harmala L. (P. harmala) is broadly used in folk medicine for the treatment of various diseases including cancer. The seeds are particularly employed for therapeutic. The present paper investigates the cytotoxic effects of raw alkaloid extracts from different parts of P. harmala including fruits (TAFr), seeds (TASe), roots (TAR) and aerial parts (TAAp); in order to assess traditional claims about the therapeutic potential of this plant. Methods: The cytotoxic effects were evaluated on six malignant cancer cells (A549, U373, Hs683, MCF7, B16F10 and SKMEL-28; A549, U373, MCF7 and SKMEL-28 are resistant to proapoptotic stimuli) by MTT assay and quantitative videomicroscopy analysis. The main alkaloids were quantified by HPLC in the different parts of plant. Results: Total alkaloids of the different parts were cytotoxic towards practically all cancer cell lines with IC 50 ranging 1-52 mg/mL after 72 h of treatment. Videomicroscopy analysis indicated that the TAFr, TASe and TAR alkaloids affect A549 lung carcinoma cells behaviour and induce a cytostatic effect whereas TAAp extract was cytotoxic rather than cytostatic. TAAp, TAFr, TASe and TAR treatment induced global growth ratio indexes (GGR) of 0.19, 0.26, 0.3 and 0.62, respectively, after 72 h of treatment. Depending on the organe, harmine, harmaline, harmol and vasicine have, range between 2 and 90% w/w of total alkaloids. Conclusion: These data indicate that P. harmala alkaloids extract may support the traditional claims regarding its anticancer uses which could be helpful in providing of new cytotoxic agents against chemoresistant cancer cells.

Disciplines :

Pharmacy, pharmacology & toxicology
Chemistry

Author, co-author :

Bournine, L

Bensalem, Sihem

Fatmi, S.

Bedjou, F

Mathieu, Véronique

Iguer-Ouada, M.

Kiss, R

Duez, Pierre ; Université de Mons > Faculté de Médecine et de Pharmacie > Service de Chimie thérapeutique et Pharmacognosie

Language :

English

Title :

Evaluation of the cytotoxic and cytostatic activities of alkaloid extracts from different parts of Peganum harmala L.(Zygophyllaceae)

Publication date :

23 January 2017

Journal title :

European Journal of Integrative Medicine

ISSN :

1876-3820

Publisher :

Elsevier, Netherlands

Volume :

Pages :

91-96

Peer reviewed :

Peer reviewed

Research unit :

M136 - Chimie thérapeutique et Pharmacognosie

Research institute :

R550 - Institut des Sciences et Technologies de la Santé

Available on ORBi UMONS :

since 23 November 2016

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