Complete biosynthesis of anthocyanins using E. coli polycultures
Authors
Andrew Jones, J.
Vernacchio, Victoria R.
Collins, Shannon M.
Shirke, Abhijit N.
Xiu, Yu
Englaender, Jacob A.
Cress, Brady F.
McCutcheon, Catherine C.
Linhardt, Robert J.
Gross, Richard A.
ORCID
https://orcid.org/0000-0003-2219-5833
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Other Contributors
Issue Date
2017-05-01
Keywords
Biology , Chemistry and chemical biology , Chemical and biological engineering , Biomedical engineering
Degree
Terms of Use
Attribution 3.0 United States
CC BY : this license allows reusers to distribute, remix, adapt, and build upon the material in any medium or format, so long as attribution is given to the creator. The license allows for commercial use. Credit must be given to the authors and the original work must be properly cited.
CC BY : this license allows reusers to distribute, remix, adapt, and build upon the material in any medium or format, so long as attribution is given to the creator. The license allows for commercial use. Credit must be given to the authors and the original work must be properly cited.
Full Citation
Complete biosynthesis of anthocyanins using E. coli polycultures J. Jones, V. Vernacchio, S. Collins, A.Shirke, Y. Xiu, J. Englaender, B, Cress, C. McCutcheon, R. J. Linhradt, R. Gross, and M. Koffas, mBio, 8, e00621-17, 2017.
Abstract
Fermentation-based chemical production strategies provide a feasible route for the rapid, safe, and sustainable production of a wide variety of important chemical products, ranging from fuels to pharmaceuticals. These strategies have yet to find wide industrial utilization due to their inability to economically compete with traditional extraction and chemical production methods. Here, we engineer for the first time the complex microbial biosynthesis of an anthocyanin plant natural product, starting from sugar. This was accomplished through the development of a synthetic, 4-strain Escherichia coli polyculture collectively expressing 15 exogenous or modified pathway enzymes from diverse plants and other microbes. This synthetic consortium-based approach enables the functional expression and connection of lengthy pathways while effectively managing the accompanying metabolic burden. The de novo production of specific anthocyanin molecules, such as calistephin, has been an elusive metabolic engineering target for over a decade. The utilization of our polyculture strategy affords milligram-per-liter production titers. This study also lays the groundwork for significant advances in strain and process design toward the development of cost-competitive biochemical production hosts through nontraditional methodologies.
Description
Complete biosynthesis of anthocyanins using E. coli polycultures
Department
The Linhardt Research Labs.
The Shirley Ann Jackson, Ph.D. Center for Biotechnology and Interdisciplinary Studies (CBIS)
The Shirley Ann Jackson, Ph.D. Center for Biotechnology and Interdisciplinary Studies (CBIS)
Publisher
American Society for Microbiology (ASM)
Relationships
The Linhardt Research Labs Online Collection
Rensselaer Polytechnic Institute, Troy, NY
mBio
https://harc.rpi.edu/
Rensselaer Polytechnic Institute, Troy, NY
mBio
https://harc.rpi.edu/
Access
A full text version is available in DSpace@RPI
CC BY — Creative Commons Attribution
CC BY — Creative Commons Attribution