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https://hdl.handle.net/20.500.14094/0100478477
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2024-06-02
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0100478477 (fulltext)
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メタデータID
0100478477
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open access
出版タイプ
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タイトル
Toll-like receptor 9 is involved in the induction of galectin-9 protein by dietary anti-allergic compound fucoidan
著者
著者名
Ezan, Gnagnan J. E.
著者ID
A1190
研究者ID
1000000212233
KUID
https://kuid-rm-web.ofc.kobe-u.ac.jp/search/detail?systemId=53bfd9d6b626d7c5520e17560c007669
著者名
Mizuno, Masashi
水野, 雅史
ミズノ, マサシ
所属機関名
農学研究科
収録物名
AIMS Allergy and Immunology
巻(号)
7(1)
ページ
24-39
出版者
AIMS Press
刊行日
2023-01-04
公開日
2023-02-13
抄録
Dietary intervention of fucoidan extracted from Saccharina japonica brown seaweed has been ascertained to favor an increase of galectin-9 protein in the intestine of allergic mice, resulting in the attenuation of the food allergy symptoms. The molecular mechanism underpinning that galectin-9 secretion remains unclear. Recently, some evidence has suggested an implication of Toll-like receptor 9 (TLR9) in galectin-9 secretion. However, no investigation has been done. For this study, we aimed to understand the relationship between galectin-9 production and fucoidan intake, which will improve the therapeutic use of fucoidan in allergy treatment. Intestinal epithelial cells (IECs) were cultured in solid or transwell plates and apically exposed to fucoidan solutions and/or synthetic TLR9 agonist (CpG-ODN). The transcriptional response of the cells to galectin-9 (lgals9) and the TLR9 gene was evaluated by using q-RTPCR, and the protein expression of galectin-9 was analyzed by conducting an ELISA test. Knockdown of TLR9 in IECs was performed by targeting TLR9 siRNA, and its effect on galectin-9 release was assessed. We found that the interaction of fucoidan and IECs resulted in the upregulation of galectin-9 released in a dose- and time-dependent manner. The increase was further potentiated in combination with the TLR9 agonist. Fucoidan exposure to IECs tended to increase the mRNA expression of TLR9 in a way similar to that of the TLR9 agonist effect, and knockdown of TLR9 in IECs resulted in a decreased tendency of fucoidan-induced galectin-9 protein. TLR9 activation is therefore involved in the increased release of galectin-9 protein observed in IECs upon fucoidan exposure.
キーワード
food allergy
dietary fucoidan
galectin-9
intestinal epithelial cells
in vitro study
Toll-like receptor 9
カテゴリ
農学研究科
学術雑誌論文
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© 2023 the Author(s), licensee AIMS Press.
This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0)
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資源タイプ
journal article
言語
English (英語)
eISSN
2575-615X
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関連情報
DOI
https://doi.org/10.3934/Allergy.2023002
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