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https://hdl.handle.net/20.500.14094/0100485455
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2024-06-02
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0100485455 (fulltext)
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0100485455
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open access
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タイトル
Nectin-4 regulates cellular senescence-associated enlargement of cell size
著者
Katasho, Ryoko ; Nagano, Taiki ; Iwasaki, Tetsushi ; Kamada, Shinji
著者名
Katasho, Ryoko
著者ID
A2799
研究者ID
1000000759988
著者名
Nagano, Taiki
長野, 太輝
ナガノ, タイキ
所属機関名
バイオシグナル総合研究センター
著者ID
A1163
研究者ID
1000040379483
KUID
https://kuid-rm-web.ofc.kobe-u.ac.jp/search/detail?systemId=58ea414cdb74786f520e17560c007669
著者名
Iwasaki, Tetsushi
岩崎, 哲史
イワサキ, テツシ
所属機関名
バイオシグナル総合研究センター
著者ID
A0461
研究者ID
1000020243214
KUID
https://kuid-rm-web.ofc.kobe-u.ac.jp/search/detail?systemId=59d2e5d38d22b1e5520e17560c007669
著者名
Kamada, Shinji
鎌田, 真司
カマダ, シンジ
所属機関名
バイオシグナル総合研究センター
収録物名
Scientific Reports
巻(号)
13(1)
ページ
21602
出版者
Nature Portfolio
刊行日
2023-12-07
公開日
2023-12-13
抄録
Cellular senescence is defined as irreversible growth arrest induced by various stress, such as DNA damage and oxidative stress. Senescent cells exhibit various characteristic morphological changes including enlarged morphology. In our recent study, we identified Nectin-4 to be upregulated in cellular senescence by comparative transcriptomic analysis. However, there are few reports on the relationship between Nectin-4 and senescence. Therefore, we analyzed the function of Nectin-4 in senescence and its biological significance. When overexpressed with Nectin-4, the cells exhibited the enlarged cell morphology closely resembling senescent cells. In addition, the cell size enlargement during DNA damage-induced senescence was suppressed by knockdown of Nectin-4, while there were no significant changes in senescence induction. These results suggest that Nectin-4 is not involved in the regulation of senescence itself but contributes to the senescence-associated cell size increase. Furthermore, the Nectin-4-dependent cell size increase was found to be mediated by Src family kinase (SFK)/PI3 kinase (PI3K)/Rac1 pathway. To explore the functional consequences of cell size enlargement, we analyzed cell survival in Nectin-4-depleted senescent cells. Single-cell tracking experiments revealed that Nectin-4 knockdown induced apoptosis in senescent cells, and there is a strong positive correlation between cell size and survival rate. These results collectively indicate that Nectin-4 plays a causative role in the senescence-associated cell size enlargement via SFK/PI3K/Rac1, which can contribute to survival of senescent cells.
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バイオシグナル総合研究センター
学術雑誌論文
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© The Author(s) 2023
This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder.
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資源タイプ
journal article
言語
English (英語)
eISSN
2045-2322
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DOI
https://doi.org/10.1038/s41598-023-48890-z
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