神戸大学附属図書館デジタルアーカイブ
入力補助
English
カテゴリ
学内刊行物
ランキング
アクセスランキング
ダウンロードランキング
https://hdl.handle.net/20.500.14094/0100486352
このアイテムのアクセス数:
32
件
(
2024-05-29
13:24 集計
)
閲覧可能ファイル
ファイル
フォーマット
サイズ
閲覧回数
説明
0100486352 (fulltext)
pdf
2.48 MB
28
メタデータ
ファイル出力
メタデータID
0100486352
アクセス権
open access
出版タイプ
Version of Record
タイトル
High-resolution structure and biochemical properties of the LH1–RC photocomplex from the model purple sulfur bacterium, Allochromatium vinosum
著者
Tani, Kazutoshi ; Kanno, Ryo ; Harada, Ayaka ; Kobayashi, Yuki ; Minamino, Akane ; Takenaka, Shinji ; Nakamura, Natsuki ; Ji, Xuan-Cheng ; Purba, Endang R. ; Hall, Malgorzata ; Yu, Long-Jiang ; Madigan, Michael T. ; Mizoguchi, Akira ; Iwasaki, Kenji ; Humbel, Bruno M. ; Kimura, Yukihiro ; Wang-Otomo, Zheng-Yu
著者名
Tani, Kazutoshi
著者名
Kanno, Ryo
著者名
Harada, Ayaka
著者名
Kobayashi, Yuki
著者名
Minamino, Akane
著者ID
A1038
研究者ID
1000040314512
KUID
https://kuid-rm-web.ofc.kobe-u.ac.jp/search/detail?systemId=c6c1a8027c337e88520e17560c007669
著者名
Takenaka, Shinji
竹中, 慎治
タケナカ, シンジ
所属機関名
農学研究科
著者名
Nakamura, Natsuki
著者名
Ji, Xuan-Cheng
著者名
Purba, Endang R.
著者名
Hall, Malgorzata
著者名
Yu, Long-Jiang
著者名
Madigan, Michael T.
著者名
Mizoguchi, Akira
著者名
Iwasaki, Kenji
著者名
Humbel, Bruno M.
著者ID
A1288
研究者ID
1000020321755
ORCID
0000-0003-3747-0367
KUID
https://kuid-rm-web.ofc.kobe-u.ac.jp/search/detail?systemId=859efd10c644420e520e17560c007669
著者名
Kimura, Yukihiro
木村, 行宏
キムラ, ユキヒロ
所属機関名
農学研究科
著者名
Wang-Otomo, Zheng-Yu
収録物名
Communications Biology
巻(号)
7(1)
ページ
176
出版者
Nature Portfolio
刊行日
2024-02-12
公開日
2024-02-26
抄録
The mesophilic purple sulfur phototrophic bacterium Allochromatium (Alc.) vinosum (bacterial family Chromatiaceae) has been a favored model for studies of bacterial photosynthesis and sulfur metabolism, and its core light-harvesting (LH1) complex has been a focus of numerous studies of photosynthetic light reactions. However, despite intense efforts, no high-resolution structure and thorough biochemical analysis of the Alc. vinosum LH1 complex have been reported. Here we present cryo-EM structures of the Alc. vinosum LH1 complex associated with reaction center (RC) at 2.24 Å resolution. The overall structure of the Alc. vinosum LH1 resembles that of its moderately thermophilic relative Alc. tepidum in that it contains multiple pigment-binding α- and β-polypeptides. Unexpectedly, however, six Ca ions were identified in the Alc. vinosum LH1 bound to certain α1/β1- or α1/β3-polypeptides through a different Ca²⁺-binding motif from that seen in Alc. tepidum and other Chromatiaceae that contain Ca²⁺-bound LH1 complexes. Two water molecules were identified as additional Ca²⁺-coordinating ligands. Based on these results, we reexamined biochemical and spectroscopic properties of the Alc. vinosum LH1–RC. While modest but distinct effects of Ca²⁺ were detected in the absorption spectrum of the Alc. vinosum LH1 complex, a marked decrease in thermostability of its LH1–RC complex was observed upon removal of Ca²⁺. The presence of Ca²⁺ in the photocomplex of Alc. vinosum suggests that Ca²⁺-binding to LH1 complexes may be a common adaptation in species of Chromatiaceae for conferring spectral and thermal flexibility on this key component of their photosynthetic machinery.
カテゴリ
農学研究科
学術雑誌論文
権利
© The Author(s) 2024.
This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder.
詳細を表示
資源タイプ
journal article
言語
English (英語)
eISSN
2399-3642
OPACで所蔵を検索
CiNiiで学外所蔵を検索
関連情報
DOI
https://doi.org/10.1038/s42003-024-05863-w
ホームへ戻る