神戸大学附属図書館デジタルアーカイブ
入力補助
English
カテゴリ
学内刊行物
ランキング
アクセスランキング
ダウンロードランキング
https://doi.org/10.24546/81000985
このアイテムのアクセス数:
4
件
(
2024-05-23
02:58 集計
)
閲覧可能ファイル
ファイル
フォーマット
サイズ
閲覧回数
説明
81000985 (fulltext)
pdf
424 KB
2
メタデータ
ファイル出力
メタデータID
81000985
アクセス権
open access
出版タイプ
Version of Record
タイトル
Diet-Induced Up-Regulation of Gene Expression in Adipocytes Without Changes in DNA Methylation
著者
Okada, Yuko ; Sakaue, Hiroshi ; Nagare, Tomoki ; Kasuga, Masato
著者名
Okada, Yuko
著者名
Sakaue, Hiroshi
著者名
Nagare, Tomoki
著者名
Kasuga, Masato
収録物名
The Kobe journal of the medical sciences
巻(号)
54(5)
ページ
241-249
出版者
神戸大学医学部
Kobe University School of Medicine
刊行日
2008
公開日
2009-04-22
抄録
The expansion of white adipose tissue (WAT) mass during the development ofobesity is mediated in part through an increase in adipocyte size. Although geneexpression profiles associated with adipogenesis in vitro and the development of obesityin vivo have been characterized by DNA microarray analysis, the role of chromatin andchromatin-modifying proteins in the regulation of gene expression related to adipocytehypertrophy has remained unclear. We have now shown that maintenance of C57BL/6Jmice on a high-fat diet for 16 weeks resulted in marked up-regulation of the expressionof leptin, Mest (mesoderm specific transcript; also known as paternally expressed gene1, or Peg1), and sFRP5 (secreted frizzled-related protein 5) genes in WAT.Furthermore, the demethylating agent 5-aza-2′-deoxycytidine increased the amount ofMest/Peg1 mRNA, but not that of leptin or sFRP5 mRNAs, in mouse 3T3-L1adipocytes. However, analysis by matrix-assisted laser desorption ionizationtime-of-flight (MALDI-TOF) mass spectrometry revealed that maintenance of mice ona high-fat diet for various times did not affect the level of methylation at specific CpGsites in the promoter regions of leptin, Mest/Peg1, and sFRP5 genes in WAT. Ourresults indicate that the diet-induced up-regulation of leptin, Mest/Peg1, and sFRP5gene expression in WAT during the development of obesity in mice is not mediateddirectly by changes in DNA methylation.
キーワード
Obesity
Adipocyte
DNA methylation
Mest/Peg1
Leptin
sFRP5
カテゴリ
The Kobe journal of the medical sciences
>
54巻
>
54巻5号(2008)
紀要論文
詳細を表示
資源タイプ
departmental bulletin paper
言語
English (英語)
ISSN
0023-2513
OPACで所蔵を検索
CiNiiで学外所蔵を検索
NCID
AA00711740
OPACで所蔵を検索
CiNiiで表示
関連情報
URI
http://www.med.kobe-u.ac.jp/journal/contents.html
ホームへ戻る