神戸大学附属図書館デジタルアーカイブ
入力補助
English
カテゴリ
学内刊行物
ランキング
アクセスランキング
ダウンロードランキング
https://doi.org/10.24546/81002596
このアイテムのアクセス数:
5
件
(
2024-05-10
20:13 集計
)
閲覧可能ファイル
ファイル
フォーマット
サイズ
閲覧回数
説明
81002596 (fulltext)
pdf
619 KB
1
メタデータ
ファイル出力
メタデータID
81002596
アクセス権
open access
出版タイプ
Version of Record
タイトル
Vitamin K2-derived Compounds Induce Growth Inhibition in Radioresistant Cancer Cells
著者
Amalia, Helfi ; Sasaki, Ryohei ; Suzuki, Yoko ; Demizu, Yusuke ; Bito, Toshinori ; Nishimura, Hideki ; Okamoto, Yoshiaki ; Yoshida, Kenji ; Miyawaki, Daisuke ; Kawabe, Tetsuya ; Mizushina, Yoshiyuki ; Sugimura, Kazuro
著者名
Amalia, Helfi
著者名
Sasaki, Ryohei
著者名
Suzuki, Yoko
著者名
Demizu, Yusuke
著者名
Bito, Toshinori
著者名
Nishimura, Hideki
著者名
Okamoto, Yoshiaki
著者名
Yoshida, Kenji
著者名
Miyawaki, Daisuke
著者名
Kawabe, Tetsuya
著者名
Mizushina, Yoshiyuki
著者名
Sugimura, Kazuro
収録物名
The Kobe journal of the medical sciences
巻(号)
56(2)
ページ
38-49
出版者
神戸大学医学部
Kobe University School of Medicine
刊行日
2010
公開日
2010-10-07
抄録
A strategy to overcome radioresistance in cancer treatment has been expected. To evaluate the strategy, appropriate experimental models are needed. Radioresistant tumour models were originally established from human colon cancer cells, and we evaluated their molecular basis. Next, the growth inhibitory effects of newly synthesized vitamin K2 (VK2)-related compounds were tested. Here, we showed that these novel compounds have growth inhibitory effects not only on cancer cells of various origins, but also on radioresistant cells, through the generation of reactive oxygen species (ROS). Human colon, lung, and breast cancer cell lines were used for testing the growth inhibitory activities of several chemical compounds. Radioresistant tumour models were established by fractionated radiation exposure. Irradiated cells were selected by a single cell cloning method, and their sensitivity to ionizing radiation was evaluated by a colony-forming assay. The VK2 derivatives (named MQ-1, MQ-2, and MQ-3) were chemically synthesized. To evaluate the generation of ROS, flow cytometer analyses were performed. A radioresistant tumour model was established from the HCT116 human colon cancer cell line. The radioresistant cells from HCT116 also showed resistance to cisplatin. In the radioresistant cells, NF-κB was highly activated. MQ-1, MQ-2, and MQ-3 showed greater growth inhibitory activities than VK2 not only in various cancer cells but also in radioresistant cells through the generation of ROS. In conclusion, a radioresistant tumour model was originally established from colon cancer cell lines through NF-κB activation, and it could be a useful tool for evaluating anti-tumour agents. Newly synthesized VK2 derivatives (MQ-1, MQ-2 and MQ-3) seemed to be potential anti-tumour agents in various cancers and radioresistant cancers. The efficacy of those compounds was related to the generation of ROS. These findings together might pave the way for the treatment of radioresistant or recurrent cancers.
カテゴリ
The Kobe journal of the medical sciences
>
56巻
>
56巻2号(2010)
紀要論文
詳細を表示
資源タイプ
departmental bulletin paper
言語
English (英語)
ISSN
0023-2513
OPACで所蔵を検索
CiNiiで学外所蔵を検索
NCID
AA00711740
OPACで所蔵を検索
CiNiiで表示
関連情報
NAID
110007670720
CiNiiで表示
URI
http://www.med.kobe-u.ac.jp/journal/contents.html
ホームへ戻る