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https://hdl.handle.net/20.500.14094/90009464
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2024-06-13
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90009464 (fulltext)
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2.69 MB
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メタデータID
90009464
アクセス権
open access
出版タイプ
Version of Record
タイトル
Role of Phosphatidylethanolamine Biosynthesis in Herpes Simplex Virus 1-Infected Cells in Progeny Virus Morphogenesis in the Cytoplasm and in Viral Pathogenicity In Vivo
著者
Arii, Jun ; Fukui, Ayano ; Shimanaka, Yuta ; Kono, Nozomu ; Arai, Hiroyuki ; Maruzuru, Yuhei ; Koyanagi, Naoto ; Kato, Akihisa ; Mori, Yasuko ; Kawaguchi, Yasushi
著者ID
A2591
研究者ID
1000030704928
KUID
https://kuid-rm-web.ofc.kobe-u.ac.jp/search/detail?systemId=1f0aa402893ddbca520e17560c007669
著者名
Arii, Jun
有井, 潤
アリイ, ジュン
所属機関名
医学研究科
著者名
Fukui, Ayano
著者名
Shimanaka, Yuta
著者名
Kono, Nozomu
著者名
Arai, Hiroyuki
著者名
Maruzuru, Yuhei
著者名
Koyanagi, Naoto
著者名
Kato, Akihisa
著者ID
A0456
研究者ID
1000050343257
KUID
https://kuid-rm-web.ofc.kobe-u.ac.jp/search/detail?systemId=11c6b67469a01145520e17560c007669
著者名
Mori, Yasuko
森, 康子
モリ, ヤスコ
所属機関名
医学研究科
著者名
Kawaguchi, Yasushi
収録物名
Journal of virology
巻(号)
94(24)
出版者
American Society for Microbiology
刊行日
2020-11-23
公開日
2022-07-08
抄録
Glycerophospholipids are major components of cell membranes. Phosphatidylethanolamine (PE) is a glycerophospholipid that is involved in multiple cellular processes, such as membrane fusion, the cell cycle, autophagy, and apoptosis. In this study, we investigated the role of PE biosynthesis in herpes simplex virus 1 (HSV-1) infection by knocking out the host cell gene encoding phosphate cytidylyltransferase 2, ethanolamine (Pcyt2), which is a key rate-limiting enzyme in one of the two major pathways for PE biosynthesis. Pcyt2 knockout reduced HSV-1 replication and caused an accumulation of unenveloped and partially enveloped nucleocapsids in the cytoplasm of an HSV-1-infected cell culture. A similar phenotype was observed when infected cells were treated with meclizine, which is an inhibitor of Pcyt2. In addition, treatment of HSV-1-infected mice with meclizine significantly reduced HSV-1 replication in the mouse brains and improved their survival rates. These results indicated that PE biosynthesis mediated by Pcyt2 was required for efficient HSV-1 envelopment in the cytoplasm of infected cells and for viral replication and pathogenicity in vivo. The results also identified the PE biosynthetic pathway as a possible novel target for antiviral therapy of HSV-associated diseases and raised an interesting possibility for meclizine repositioning for treatment of these diseases, since it is an over-the-counter drug that has been used for decades against nausea and vertigo in motion sickness.
キーワード
envelopment
herpes simplex virus
phospholipids
viral pathogenesis
カテゴリ
医学研究科
学術雑誌論文
権利
©2020 AMERICAN SOCIETY FOR MICROBIOLOGY.
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資源タイプ
journal article
言語
English (英語)
ISSN
0022-538x
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eISSN
1098-5514
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関連情報
DOI
https://doi.org/10.1128/JVI.01572-20
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