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Extended depth of field imaging for high speed object analysisA high speed, high-resolution flow imaging system is modified to achieve extended depth of field imaging. An optical distortion element is introduced into the flow imaging system. Light from an object, such as a cell, is distorted by the distortion element, such that a point spread function (PSF) of the imaging system is invariant across an extended depth of field. The distorted light is spectrally dispersed, and the dispersed light is used to simultaneously generate a plurality of images. The images are detected, and image processing is used to enhance the detected images by compensating for the distortion, to achieve extended depth of field images of the object. The post image processing preferably involves de-convolution, and requires knowledge of the PSF of the imaging system, as modified by the optical distortion element.
Document ID
20110015432
Acquisition Source
Ames Research Center
Document Type
Other - Patent
Authors
Ortyn, William
Basiji, David
Frost, Keith
Liang, Luchuan
Bauer, Richard
Hall, Brian
Perry, David
Date Acquired
August 25, 2013
Publication Date
August 23, 2011
Subject Category
Instrumentation And Photography
Funding Number(s)
CONTRACT_GRANT: NNA05CR09C
Distribution Limits
Public
Copyright
Work of the US Gov. Public Use Permitted.
Patent
US-Patent-8,005,314
Patent Application
US-Patent-Appl-SN-11/609,269

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