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Book Chapter

Patch pipettes used for loading small cells with fluorescent indicator dyes

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Neher,  E.
Department of Membrane Biophysics, MPI for biophysical chemistry, Max Planck Society;

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Almers,  W.
Department of Membrane Biophysics, MPI for biophysical chemistry, Max Planck Society;

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Citation

Neher, E., & Almers, W. (1987). Patch pipettes used for loading small cells with fluorescent indicator dyes. In I. Atwater, E. Rojas, & B. Soria (Eds.), Biophysics of the Pankreatic ß-Cells (pp. 1-5). Boston MA: Springer.


Cite as: https://hdl.handle.net/21.11116/0000-0003-EA8D-7
Abstract
In modern cell physiology, much important information has come from the intracellular application of membrane-impermeant substances, such as ion indicator dyes, second messengers, regulatory proteins, antibodies and messenger RNA. Such substances have been either injected into cells through microelectrodes or loaded into lipid vesicles and erythrocyte ghosts that were later allowed to fuse with the cell membrane. We have monitored cytoplasmic Ca2+ in mast cells with intracellularly applied fura-2, a new Ca-indicator dye.