Deutsch
 
Hilfe Datenschutzhinweis Impressum
  DetailsucheBrowse

Datensatz

DATENSATZ AKTIONENEXPORT

Freigegeben

Zeitschriftenartikel

Bacterially Derived Antibody Binders as Small Adapters for DNA‐PAINT Microscopy

MPG-Autoren
/persons/resource/persons197581

Schlichthaerle,  Thomas
Jungmann, Ralf / Molecular Imaging and Bionanotechnology, Max Planck Institute of Biochemistry, Max Planck Society;

/persons/resource/persons229346

Ganji,  Mahipal
Jungmann, Ralf / Molecular Imaging and Bionanotechnology, Max Planck Institute of Biochemistry, Max Planck Society;

/persons/resource/persons209115

Auer,  Alexander
Jungmann, Ralf / Molecular Imaging and Bionanotechnology, Max Planck Institute of Biochemistry, Max Planck Society;

/persons/resource/persons209108

Wade,  Orsolya Kimbu
Jungmann, Ralf / Molecular Imaging and Bionanotechnology, Max Planck Institute of Biochemistry, Max Planck Society;

/persons/resource/persons172959

Jungmann,  Ralf
Jungmann, Ralf / Molecular Imaging and Bionanotechnology, Max Planck Institute of Biochemistry, Max Planck Society;

Volltexte (beschränkter Zugriff)
Für Ihren IP-Bereich sind aktuell keine Volltexte freigegeben.
Volltexte (frei zugänglich)
Ergänzendes Material (frei zugänglich)
Es sind keine frei zugänglichen Ergänzenden Materialien verfügbar
Zitation

Schlichthaerle, T., Ganji, M., Auer, A., Wade, O. K., & Jungmann, R. (2019). Bacterially Derived Antibody Binders as Small Adapters for DNA‐PAINT Microscopy. ChemBioChem: A European Journal of Chemical Biology, 20(8), 1032-1038. doi:10.1002/cbic.201800743.


Zitierlink: https://hdl.handle.net/21.11116/0000-0003-E68E-A
Zusammenfassung
Current optical super-resolution implementations are capable of resolving features spaced just a few nanometers apart. However, translating this spatial resolution to cellular targets is limited by the large size of traditionally employed primary and secondary antibody reagents. Recent advancements in small and efficient protein binders for super-resolution microscopy such as nanobodies or aptamers provide an exciting avenue for the future, however their widespread availability is still limited. To address this issue, we here report the combination of bacterial-derived binders commonly used in antibody purification with DNA-PAINT microscopy. The small size of these protein binders compared to secondary antibodies make them an attractive labeling alternative for emerging super-resolution techniques. We here present a labeling protocol for DNA conjugation of bacterial-derived protein A and G for DNA-PAINT imaging and assay their performance intracellularly by targeting primary antibodies against Tubulin, TOM20, and EGFR and quantify the increase in obtainable resolution. © 2018 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.