Illumination guidelines for ultrafast pump–probe experiments by serial 
femtosecond crystallography

Grünbein, Marie Luise; Stricker, Miriam; Nass Kovács, Gabriela; Kloos, Marco; Doak, Bruce; Shoeman, Robert L.; Reinstein, Jochen; Lecler , Sylvain; Haacke , Stefan; Schlichting, Ilme

doi:10.1038/s41592-020-0847-3

アイテム詳細

登録内容を編集ファイル形式で保存

一時保存へ追加

タグ情報を表示リリース履歴を表示詳細要約

公開

学術論文

Illumination guidelines for ultrafast pump–probe experiments by serial femtosecond crystallography

MPS-Authors

/persons/resource/persons123592

Grünbein, Marie Luise
Department of Biomolecular Mechanisms, Max Planck Institute for Medical Research, Max Planck Society;

/persons/resource/persons225677

Stricker, Miriam
Department of Biomolecular Mechanisms, Max Planck Institute for Medical Research, Max Planck Society;

/persons/resource/persons183396

Nass Kovács, Gabriela
Department of Biomolecular Mechanisms, Max Planck Institute for Medical Research, Max Planck Society;

/persons/resource/persons203860

Kloos, Marco
Department of Biomolecular Mechanisms, Max Planck Institute for Medical Research, Max Planck Society;

/persons/resource/persons117878

Doak, Bruce
Department of Biomolecular Mechanisms, Max Planck Institute for Medical Research, Max Planck Society;

/persons/resource/persons95345

Shoeman, Robert L.
Department of Biomolecular Mechanisms, Max Planck Institute for Medical Research, Max Planck Society;

/persons/resource/persons94928

Reinstein, Jochen
Department of Biomolecular Mechanisms, Max Planck Institute for Medical Research, Max Planck Society;

/persons/resource/persons95189

Schlichting, Ilme
Department of Biomolecular Mechanisms, Max Planck Institute for Medical Research, Max Planck Society;

External Resource

https://www.nature.com/articles/s41592-020-0847-3.pdf
(全文テキスト（全般）)

https://static-content.springer.com/esm/art%3A10.1038%2Fs41592-020-0847-3/MediaObjects/41592_2020_847_MOESM1_ESM.pdf
(付録資料)

https://doi.org/10.1038/s41592-020-0847-3
(全文テキスト（全般）)

Fulltext (restricted access)

There are currently no full texts shared for your IP range.

フルテキスト (公開)

公開されているフルテキストはありません

付随資料 (公開)

There is no public supplementary material available

引用

Grünbein, M. L., Stricker, M., Nass Kovács, G., Kloos, M., Doak, B., Shoeman, R. L., Reinstein, J., Lecler, S., Haacke, S., & Schlichting, I. (2020). Illumination guidelines for ultrafast pump–probe experiments by serial femtosecond crystallography. Nature methods, 17(7), 681-684. doi:10.1038/s41592-020-0847-3.

引用: https://hdl.handle.net/21.11116/0000-0006-7007-4

要旨

Time-resolved crystallography with X-ray free-electron lasers enables structural characterization of light-induced reactions on ultrafast timescales. To be biologically and chemically relevant, such studies must be carried out in an appropriate photoexcitation regime to avoid multiphoton artifacts, a common issue in recent studies. We describe numerical and experimental approaches to determine how many photons are needed for single-photon excitation in microcrystals, taking into account losses by scattering.