Nucleo-cytoplasmic flux and intracellular mobility in single hepatocytes 
measured by fluorescence microphotolysis

Peters, Reiner

doi:10.1002/j.1460-2075.1984.tb02055.x

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Nucleo-cytoplasmic flux and intracellular mobility in single hepatocytes measured by fluorescence microphotolysis

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Peters, Reiner
Department of Cell Physiology, Max Planck Institute of Biophysics, Max Planck Society;

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引用

Peters, R. (1984). Nucleo-cytoplasmic flux and intracellular mobility in single hepatocytes measured by fluorescence microphotolysis. EMBO Journal, 3(8), 1831-1836. doi:10.1002/j.1460-2075.1984.tb02055.x.

引用: https://hdl.handle.net/21.11116/0000-0008-05AF-E

要旨

Fluorescence microphotolysis was used to measure nucleocytoplasmic flux in single rat hepatocytes for a series of dextrans ranging in molecular mass from 3 to 150 kd. The cytoplasmic translational diffusion coefficient D_Cand the nucleoplasmic diffusion coefficient D_N of a 62-kd dextran were also determined. D_C was approximately 2 X 10^-8 and D_N approximately 3 X 10^-8 cm²/s, i.e., 1/20-1/15 of the value in free solution. The mobile fraction amounted to 0.7-0.8 in measurements of both intracellular diffusion and nucleo-cytoplasmic flux. The flux of dextrans from cytoplasm to nucleus depended inversely on molecular mass with an exclusion limit between 17 and 41 kd suggesting that the nuclear envelope has functions of a molecular sieve. Employing the Pappenheimer-Renkin equations, a functional pore radius of 50-56 Å was derived. By comparison with recent measurements on isolated liver cell nuclei, large quantitative differences between the intracellularly located and the isolated nucleus were revealed.