LSm1-7 complexes bind to specific sites in viral RNA genomes and regulate their 
translation and replication

Galão, R. P.; Chari, A.; Alves-Rodrigues, I.; Lobão, D.; Mas, A.; Kambach, C.; Fischer, U.; Díez, J.

doi:10.1261/rna.1712910

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LSm1-7 complexes bind to specific sites in viral RNA genomes and regulate their translation and replication

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Chari, A.
Research Group of Structural Biochemistry and Mechanisms, MPI for Biophysical Chemistry, Max Planck Society;

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Citation

Galão, R. P., Chari, A., Alves-Rodrigues, I., Lobão, D., Mas, A., Kambach, C., et al. (2010). LSm1-7 complexes bind to specific sites in viral RNA genomes and regulate their translation and replication. RNA, 16(4), 817-827. doi:10.1261/rna.1712910.

Cite as: https://hdl.handle.net/21.11116/0000-0008-08D3-1

Abstract

LSm1-7 complexes promote cellular mRNA degradation, in addition to translation and replication of positive-strand RNA viruses such as the Brome mosaic virus (BMV). Yet, how LSm1-7 complexes act on their targets remains elusive. Here, we report that reconstituted recombinant LSm1-7 complexes directly bind to two distinct RNA-target sequences in the BMV genome, a tRNA-like structure at the 3′-untranslated region and two internal A-rich single-stranded regions. Importantly, in vivo analysis shows that these sequences regulate the translation and replication of the BMV genome. Furthermore, both RNA-target sequences resemble those found for Hfq, the LSm counterpart in bacteria, suggesting conservation through evolution. Our results provide the first evidence that LSm1-7 complexes interact directly with viral RNA genomes and open new perspectives in the understanding of LSm1-7 functions.