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Complete Freund's adjuvant-free experimental autoimmune encephalomyelitis in Dark Agouti rats is a valuable tool for multiple sclerosis studies

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Krishnamoorthy,  Gurumoorthy
Krishnamoorthy, Gurumoorthy / Neuroinflammation and Mucosal Immunology, Max Planck Institute of Biochemistry, Max Planck Society;

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Citation

Lazarevic, M., Djedovic, N., Stanisavljevic, S., Dimitrijevic, M., Stegnjaic, G., Krishnamoorthy, G., et al. (2021). Complete Freund's adjuvant-free experimental autoimmune encephalomyelitis in Dark Agouti rats is a valuable tool for multiple sclerosis studies. Journal of Neuroimmunology, 354: 577547. doi:10.1016/j.jneuroim.2021.577547.


Cite as: https://hdl.handle.net/21.11116/0000-0008-A3A1-9
Abstract
Experimental autoimmune encephalomyelitis (EAE) is classically induced with complete Freund's adjuvant (CFA). The immune response against CFA has a confounding influence on the translational capacity of EAE as a multiple sclerosis model. Here, we compare clinical, cellular and molecular properties between syngeneic spinal cord homogenate (SCH)- and SCH + CFA-immunized Dark Agouti rats. EAE signs were observed earlier and the cumulative clinical score was higher without CFA. Also, a higher number of immune cells infiltrates in the spinal cords was noticed at the peak of EAE without CFA. High spinal cord abundance of CD8(+)CD11bc(+)MHC class II+ cells was detected in SCH-immunized rats. Myelin basic protein -specific response can be elicited in the cells from the lymph nodes draining the site of SCH immunization. This CFA-free EAE is a reliable multiple sclerosis model.