Yeast Homologous Recombination Cloning Leading to the Novel Peptides Ambactin 
and Xenolindicin

Schimming, Olivia; Fleischhacker, Florian; Nollmann, Friederike I.; Bode, Helge B.

doi:10.1002/cbic.201402065

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Yeast Homologous Recombination Cloning Leading to the Novel Peptides Ambactin and Xenolindicin

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Zitation

Schimming, O., Fleischhacker, F., Nollmann, F. I., & Bode, H. B. (2014). Yeast Homologous Recombination Cloning Leading to the Novel Peptides Ambactin and Xenolindicin. CHEMBIOCHEM, 15(9), 1290-1294. doi:10.1002/cbic.201402065.

Zitierlink: https://hdl.handle.net/21.11116/0000-000A-04B6-4

Zusammenfassung

Heterologous production of GameXPeptide A (1), as well as of the novel peptide natural products ambactin (2) and xenolindicins A-C (3a-c), was achieved by using the "overlap extension PCR-yeast homologous recombination" (ExRec) method. ExRec cloning is based on the ability of yeast to assemble overlapping DNA fragments into functional plasmids. Here we used this technique to clone a total of 15 biosynthesis gene clusters from Photorhabdus and Xenorhabdus with sizes of up to 45 kb. The structures of the novel compounds 2 and 3a, which were produced in Escherichia coli, were elucidated by detailed MS and bioinformatics analysis, and additionally confirmed by their chemical synthesis.