Assessing Distribution and Variation of Genome-Wide DNA Methylation Using 
Short-Read Sequencing

Hagmann, J; Becker, C

doi:10.1007/978-1-4939-7003-2_5

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Assessing Distribution and Variation of Genome-Wide DNA Methylation Using Short-Read Sequencing

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Becker, C
Department Molecular Biology, Max Planck Institute for Developmental Biology, Max Planck Society;

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引用

Hagmann, J., & Becker, C. (2017). Assessing Distribution and Variation of Genome-Wide DNA Methylation Using Short-Read Sequencing. Methods in Molecular Biology, 1610. doi:10.1007/978-1-4939-7003-2_5.

引用: https://hdl.handle.net/21.11116/0000-0002-04B6-C

要旨

DNA methylation is one of the most prominent epigenetic marks and is particularly complex in plant genomes. Whole-genome bisulfite sequencing (WGBS) using short reads has become the standard tool to study genome-wide patterns of DNA methylation. The goal of the present protocol is to enable readers to perform WGBS on both the wet lab and the computational side. We briefly outline important steps in bisulfite library preparation, then focus on the different aspects of DNA methylation analysis, from read mapping to identifying biologically relevant differential methylation between different samples.