DENTIST-using long reads for closing assembly gaps at high accuracy.

Ludwig, Arne; Pippel, Martin; Myers, Gene; Hiller, Michael

doi:10.1093/gigascience/giab100

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DENTIST-using long reads for closing assembly gaps at high accuracy.

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Pippel, Martin
Max Planck Institute for Molecular Cell Biology and Genetics, Max Planck Society;

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Myers, Gene
Max Planck Institute for Molecular Cell Biology and Genetics, Max Planck Society;

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Hiller, Michael
Max Planck Institute for Molecular Cell Biology and Genetics, Max Planck Society;

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引用

Ludwig, A., Pippel, M., Myers, G., & Hiller, M. (2022). DENTIST-using long reads for closing assembly gaps at high accuracy. GigaScience, 11:. doi:10.1093/gigascience/giab100.

引用: https://hdl.handle.net/21.11116/0000-000B-0333-8

要旨

Long sequencing reads allow increasing contiguity and completeness of fragmented, short-read-based genome assemblies by closing assembly gaps, ideally at high accuracy. While several gap-closing methods have been developed, these methods often close an assembly gap with sequence that does not accurately represent the true sequence.