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Near-infrared STED nanoscopy with an engineered bacterial phytochrome

MPG-Autoren
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Kamper,  M.
Department of NanoBiophotonics, MPI for Biophysical Chemistry, Max Planck Society;

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Ta,  H.
Department of NanoBiophotonics, MPI for Biophysical Chemistry, Max Planck Society;

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Jensen,  N. A.
Department of NanoBiophotonics, MPI for Biophysical Chemistry, Max Planck Society;

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Hell,  S. W.       
Department of NanoBiophotonics, MPI for Biophysical Chemistry, Max Planck Society;

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Jakobs,  S.
Research Group of Mitochondrial Structure and Dynamics, MPI for biophysical chemistry, Max Planck Society;

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Zitation

Kamper, M., Ta, H., Jensen, N. A., Hell, S. W., & Jakobs, S. (2018). Near-infrared STED nanoscopy with an engineered bacterial phytochrome. Nature Communications, 9: 4762. doi:10.1038/s41467-018-07246-2.


Zitierlink: https://hdl.handle.net/21.11116/0000-0002-7E20-D
Zusammenfassung
The near infrared (NIR) optical window between the cutoff for hemoglobin absorption at 650 nm and the onset of increased water absorption at 900 nm is an attractive, yet largely unexplored, spectral regime for diffraction-unlimited super-resolution fluorescence microscopy (nanoscopy). We developed the NIR fluorescent protein SNIFP, a bright and photostable bacteriophytochrome, and demonstrate its use as a fusion tag in live-cell microscopy and STED nanoscopy. We further demonstrate dual color red-confocal/NIR-STED imaging by co-expressing SNIFP with a conventional red fluorescent protein.