Extraction of high-quality genomic DNA from Ectocarpus

Coelho, SM; Scornet, D; Rousvoal, S; Peters, N; Dartevelle , L; Peters, AF; Cock, JM

doi:10.1101/pdb.prot067967

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Extraction of high-quality genomic DNA from Ectocarpus

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Coelho, S., Scornet, D., Rousvoal, S., Peters, N., Dartevelle, L., Peters, A., et al. (2012). Extraction of high-quality genomic DNA from Ectocarpus. Cold Spring Harbor protocols, 2012(3), 365-368. doi:10.1101/pdb.prot067967.

Cite as: https://hdl.handle.net/21.11116/0000-000C-7C42-F

Abstract

For some applications, such as genome sequencing and high-throughput genotyping with multiple markers, it is necessary to use high-quality genomic DNA. This article describes how to obtain several micrograms of high-quality, cesium chloride-purified DNA from 1 g of Ectocarpus filaments. We also recommend using DNA of this quality for quantitative RT-PCR control reactions. However, simpler, more rapid, kit-based methods are preferable for experiments that involve the treatment of large numbers of individuals, such as genotyping large populations with a small number of markers or PCR screening of large populations.