Evaluation of a thermophilic, psychrostable, and heavy metal-resistant Red Sea 
brine pool esterase

Ahmed, Shimaa F.; Abdallah, Rehab Z.; Siam, Rania

doi:10.3390/md20050274

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Evaluation of a thermophilic, psychrostable, and heavy metal-resistant Red Sea brine pool esterase

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Abdallah, Rehab Z.
Max Planck Institute for Terrestrial Microbiology, Max Planck Society;

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https://doi.org/10.3390/md20050274
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Citation

Ahmed, S. F., Abdallah, R. Z., & Siam, R. (2022). Evaluation of a thermophilic, psychrostable, and heavy metal-resistant Red Sea brine pool esterase. Marine Drugs, 20(5): 274. doi:10.3390/md20050274.

Cite as: https://hdl.handle.net/21.11116/0000-000A-9CDB-0

Abstract

Lipolytic enzymes catalyze the hydrolysis and synthesis of ester compounds. They are valuable in the pulp, food, and textile industries. This study aims to comprehensively evaluate the extreme properties of a hormone-sensitive lipase (EstATII-TM) isolated from the Red Sea Atlantis II brine pool. EstATII-TM was cloned, expressed, and its biochemical activities were assessed under different conditions. EstATII-TM catalytic properties and resistance to different metal ions were compared to commercial thermophilic esterases under different temperatures. Phylogenetically, EstATII-TM was assigned to the GDSAG motif subfamily of hormone-sensitive lipase. The optimal enzyme activity was evident at a temperature of 30 degrees C and pH 7-8. The enzyme retained 84.9% of its activity at 0.5 M NaCl. EstATII-TM maintained 93% to 97% activity at -40 and -20 degrees C, respectively. EstATII-TM activity was significantly enhanced, up to 10-fold, at temperatures ranging from 45 to 65 degrees C in the presence of 1 mM Cu2+, Cd2+, Ba2+, Mn2+, and Zn2+. EstATII-TM showed superior catalytic activity and resistance-to/enhancement-by metal ions compared to two commercial thermophilic esterases. The Red Sea Atlantis II brine EstATII-TM is characterized by tolerance to high temperatures, stability to hot and cold conditions, as well as toxic heavy metal contamination, making it an ideal candidate for industrial processes.