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Physical and functional interactions between STAP-2/BKS and STAT5.
| Title: | Physical and functional interactions between STAP-2/BKS and STAT5. |
| Authors: | Sekine, Yuichi Browse this author | | Yamamoto, Tetsuya Browse this author | | Yumioka, Taro Browse this author | | Sugiyama, Kenji Browse this author | | Tsuji, Satoshi Browse this author | | Oritani, Kenji Browse this author | | Shimoda, Kazuya Browse this author | | Minoguchi, Mayu Browse this author | | Yoshimura, Akihiko Browse this author | | Matsuda, Tadashi Browse this author →KAKEN DB |
| Issue Date: | 4-Mar-2005 |
| Publisher: | American Society for Biochemistry and Molecular Biology |
| Journal Title: | Journal of Biological Chemistry |
| Volume: | 280 |
| Issue: | 9 |
| Start Page: | 8188 |
| End Page: | 8196 |
| Publisher DOI: | 10.1074/jbc.M411692200 |
| PMID: | 15611091 |
| Abstract: | Signal-transducing adaptor protein family of proteins (STAPs), which currently contains two members, are proposed to be adaptor molecules because of their pleckstrin homology (PH) and Src-homology 2 (SH2)-like domains. STAP-1 has been shown to interact with STAT5 and the tyrosine kinase Tec. With regard to STAP-2/BKS functions, immunoprecipitation experiments and intracellular stainings revealed STAP-2/BKS binds STAT5 in several types of cells. Mutational studies revealed that the PH- and SH2-like domains of STAP-2/BKS interacted with the C-terminal region of STAT5. STAP-2/BKS and STAT5 were found to constitutively co-localize in the cytoplasm of resting cells, but STAP-2/BKS was found to dissociate upon STAT5 phosphorylation, suggesting a role in regulating signaling of STAT5. The physiological role of these interactions is not fully understood, but in studies of overexpression of STAP-2/BKS, cytokine-induced tyrosine phosphorylation and transcriptional activation of STAT5 was diminished. In addition, thymocytes from STAP-2/BKS-deficient mice showed the enhanced interleukin-2-dependent cell growth. Taken together, STAP-2/BKS is an additional modulator of STAT5-mediated signaling. |
| Rights: | Copyright © 2005 by the American Society for Biochemistry and Molecular Biology |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/28113 |
| Appears in Collections: | 薬学研究院 (Faculty of Pharmaceutical Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 松田 正
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