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Immune-mediated antitumor effect of a transplanted lymph node
| Title: | Immune-mediated antitumor effect of a transplanted lymph node |
| Authors: | Maeda, Taku Browse this author | | Hayashi, Toshihiko Browse this author | | Furukawa, Hiroshi Browse this author →KAKEN DB | | Iwasaki, Daisuke Browse this author | | Ishikawa, Kosuke Browse this author | | Funayama, Emi Browse this author | | Murao, Naoki Browse this author →KAKEN DB | | Osawa, Masayuki Browse this author | | Oyama, Akihiko Browse this author | | Yamamoto, Yuhei Browse this author →KAKEN DB |
| Keywords: | antitumor effect | | lymph node transplantation | | lymphatic system | | melanoma | | tumor-draining lymph node |
| Issue Date: | 1-Sep-2018 |
| Publisher: | John Wiley & Sons |
| Journal Title: | International journal of cancer |
| Volume: | 143 |
| Issue: | 5 |
| Start Page: | 1224 |
| End Page: | 1235 |
| Publisher DOI: | 10.1002/ijc.31414 |
| Abstract: | Lymph node (LN) transplantation is a recognized method for reconstruction of the lymphatic system and is used in the clinical setting to treat lymphedema. However, it is unclear whether transplanted LNs contribute to immune surveillance. In our study, we investigated whether a single transplanted non-vascularized LN, defined as a tumor-draining transplanted lymph node (TDTLN), could exert an immune-mediated antitumor effect. LN and lung metastases and primary tumor enlargement were evaluated in mice that were inoculated with B16-F10-luc2 melanoma cells in a hind limb footpad without (group 1) and with (group 2) popliteal lymph node (PLN) resection and in mice that underwent LN transplantation after PLN resection (group 3). The function of a TDTLN (group 3) and a tumor-draining popliteal lymph node (TDPLN; group 1) was evaluated in the context of cancer. LN and lung metastases were significantly aggravated by PLN resection but were significantly decreased by LN transplantation. Immunohistochemistry showed that the TDTLNs retained T-cells and B-cells and fluorescence-activated cell sorting analysis confirmed expansion of lymphocytes in these nodes; however, the degree of expansion in TDTLNs was different from that in TDPLNs. Expression of cytokines associated with immunostimulation was confirmed in the TDTLNs as well as in the TDPLNs. One of the differences in the immune-mediated antitumor effect of the TDPLNs and TDTLNs was ascribed to a difference in the site of lymphocyte homing to peripheral LNs through high endothelial venules. Non-vascularized LN transplantation had an immune-mediated antitumor effect. |
| Rights: | This is the peer reviewed version of the following article: Maeda, T., Hayashi, T., Furukawa, H. Iwasaki, D., Ishikawa, K., Funayama, E., Murao, N., Osawa, M., Oyama, A. and Yamamoto, Y. (2018), Immune‐mediated antitumor effect of a transplanted lymph node. Int. J. Cancer, 143: 1224-1235., which has been published in final form at https://doi.org/10.1002/ijc.31414. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions. |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/75318 |
| Appears in Collections: | 医学院・医学研究院 (Graduate School of Medicine / Faculty of Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 前田 拓
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