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Critical evaluation of the interaction of reactive oxygen and nitrogen species with blood to inform the clinical translation of nonthermal plasma therapy
journal contribution
posted on 2021-01-22, 14:56 authored by Abraham Lin, Eline Biscop, Colum Breen, Stephen ButlerStephen Butler, Evelien Smits, Annemie BogaertsNon-thermal plasma (NTP), an ionized gas generated at ambient pressure and temperature, has been an emerging technology for medical applications. Through controlled delivery of reactive oxygen and nitrogen species (ROS/RNS), NTP can elicit hormetic cellular responses, thus stimulating broad therapeutic effects. To enable clinical translation of the promising preclinical research into NTP therapy, a deeper understanding of NTP interactions with clinical substrates is profoundly needed. Since NTP-generated ROS/RNS will inevitably interact with blood in several clinical contexts, understanding their stability in this system is crucial. In this study, two medically relevant NTP delivery modalities were used to assess the stability of NTP-generated ROS/RNS in three aqueous solutions with increasing organic complexities: phosphate-buffered saline (PBS), blood plasma (BP), and processed whole blood. NTP-generated RNS collectively (NO2−, ONOO−), H2O2, and ONOO− exclusively were analyzed over time. We demonstrated that NTP-generated RNS and H2O2 were stable in PBS but scavenged by different components of the blood. While RNS remained stable in BP after initial scavenging effects, it was completely reduced in processed whole blood. On the other hand, H2O2 was completely scavenged in both liquids over time. Our previously developed luminescent probe europium(III) was used for precision measurement of ONOO− concentration. NTP-generated ONOO− was detected in all three liquids for up to at least 30 seconds, thus highlighting its therapeutic potential. Based on our results, we discussed the necessary considerations to choose the most optimal NTP modality for delivery of ROS/RNS to and via blood in the clinical context.
Funding
Research Foundation Flanders grant 12S9218N,12S9221N and G044420N
Methusalem grant
History
School
- Science
Department
- Chemistry
Published in
Oxidative Medicine and Cellular LongevityVolume
2020Publisher
Hindawi LimitedVersion
- VoR (Version of Record)
Rights holder
© The AuthorsPublisher statement
This is an Open Access Article. It is published by Hindawi under the Creative Commons Attribution 4.0 International Licence (CC BY 4.0). Full details of this licence are available at: https://creativecommons.org/licenses/by/4.0/Acceptance date
2020-11-04Publication date
2020-12-03Copyright date
2020ISSN
1942-0900eISSN
1942-0994Publisher version
Language
- en
Depositor
Dr Stephen Butler. Deposit date: 20 January 2021Article number
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