membranes-11-00257.pdf (3.72 MB)
Effects of scaffold pore morphologies on glucose transport limitations in hollow fibre membrane bioreactor for bone tissue engineering: experiments and numerical modelling
journal contribution
posted on 2021-04-08, 12:55 authored by Simon WangSimon Wang, Hazwani Suhaimi, Mostafa Mabrouk, Stella GeorgiadouStella Georgiadou, John WardJohn Ward, Diganta DasDiganta DasIn the current research, three electrospun polycaprolactone (PCL) scaffolds with different
pore morphology induced by changing the electrospinning parameters, spinning time and rate, have
been prepared in order to provide a fundamental understanding on the effects pore morphology have
on nutrient transport behaviour in hollow fibre membrane bioreactor (HFMB). After determining
the porosity of the scaffolds, they were investigated for glucose diffusivity using cell culture media
(CCM) and distilled water in a diffusion cell at 37 ◦C. The scanning electron microscope (SEM) images
of the microstructure of the scaffolds were analysed further using ImageJ software to determine
the porosity and glucose diffusivity. A Krogh cylinder model was used to determine the glucose
transport profile with dimensionless variables within the HFMB. The paper discusses the roles of
various dimensionless numbers (e.g., Péclet and Damköhler numbers) and non-dimensional groups
of variables (e.g., non-dimensional fibre radius) on determining glucose concentration profiles,
especially in the scaffold region. A negative linear relationship between glucose diffusivities across
PCL scaffolds and the minimum glucose concentrations (i.e., concentration on the outer fibre edge
on the outlet side (at z = 1 and r = 3.2) was also found. It was shown that the efficiency of glucose
consumption improves with scaffolds of higher diffusivities. The results of this study are expected
to help in optimizing designs of HFMB as well as carry out more accurate up scaling analyses for
the bioreactor
History
School
- Aeronautical, Automotive, Chemical and Materials Engineering
Department
- Chemical Engineering
Published in
MembranesVolume
11Issue
4Publisher
MDPI AGVersion
- VoR (Version of Record)
Rights holder
© The authorsPublisher statement
This is an Open Access Article. It is published by MDPI under the Creative Commons Attribution 4.0 Unported Licence (CC BY). Full details of this licence are available at: http://creativecommons.org/licenses/by/4.0/Acceptance date
2021-03-31Publication date
2021-04-02Copyright date
2021ISSN
2077-0375Publisher version
Language
- en
Depositor
Dr Diganta Das. Deposit date: 2 April 2021Article number
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