Article (Scientific journals)
New asthma biomarkers: lessons from murine models of acute and chronic asthma.
Di Valentin, Emmanuel; Crahay, Céline; Garbacki, Nancy et al.
2009In American Journal of Physiology - Lung Cellular and Molecular Physiology, 296 (2), p. 185-97
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Keywords :
microarray; ovalbumin-induced asthma; airway inflammation; remodeling
Abstract :
[en] Many patients suffering from asthma are not fully controlled by currently available treatments, and some of them display an airway remodeling leading to exaggerated lung function decline. The aim of the present study was to unveil new mediators in asthma to better understand pathophysiology and propose or validate new potential therapeutic targets. A mouse model of asthma mimicking acute or chronic asthma disease was used to select genes undergoing a modulation in both acute and chronic conditions. Mice were exposed to ovalbumin or PBS for 1, 5, and 10 wk [short-, intermediate-, and long-term model (ST, IT, and LT)], and gene expression in the lung was studied using an Affymetrix 430 2.0 genome-wide microarray and further confirmed by RT-PCR and immunohistochemistry for selected targets. We report that 598, 1,406, and 117 genes were upregulated and 490, 153, 321 downregulated at ST, IT, and LT, respectively. Genes related to mucous secretion displayed a progressively amplified expression during the allergen exposure protocol, whereas genes corresponding to growth and differentiation factors, matrix metalloproteinases, and collagens were mainly upregulated at IT. By contrast, genes related to cell division were upregulated at ST and IT and were downregulated at LT. In this study, besides confirming that Arg1, Slc26a4, Ear11, and Mmp12 genes are highly modulated throughout the asthma pathology, we show for the first time that Agr2, Scin, and Cd209e genes are overexpressed throughout the allergen exposure and might therefore be considered as suitable new potential targets for the treatment of asthma.
Research center :
Giga-Cancer - ULiège
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Di Valentin, Emmanuel  ;  Université de Liège - ULiège > GIGA-R : Virologie - Immunologie
Crahay, Céline
Garbacki, Nancy  ;  Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Protéines et glycoprot. de matr.extracell. et membran.basal.
Hennuy, Benoît ;  Université de Liège - ULiège > GIGA-Management : Plate-forme transcriptomique
Guéders, Maud ;  Université de Liège - ULiège > Département des sciences cliniques > Labo de biologie des tumeurs et du développement
Noël, Agnès ;  Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Biologie cellulaire et moléculaire appliquée à l'homme
Foidart, Jean-Michel ;  Université de Liège - ULiège > Département des sciences cliniques > Gynécologie - Obstétrique - Labo de biologie des tumeurs et du développement
Grooten, J.
Colige, Alain ;  Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Protéines et glycoprot. de matr.extracell. et membran.basal.
Piette, Jacques ;  Université de Liège - ULiège > Département des sciences de la vie > GIGA-R : Virologie - Immunologie - GIGA-Research
Cataldo, Didier  ;  Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Biochimie et physiologie générales, humaines et path.
Language :
English
Title :
New asthma biomarkers: lessons from murine models of acute and chronic asthma.
Publication date :
February 2009
Journal title :
American Journal of Physiology - Lung Cellular and Molecular Physiology
ISSN :
1040-0605
eISSN :
1522-1504
Publisher :
American Physiological Society, Bethesda, United States - Maryland
Volume :
296
Issue :
2
Pages :
L185-97
Peer reviewed :
Peer Reviewed verified by ORBi
Funders :
F.R.S.-FNRS - Fonds de la Recherche Scientifique [BE]
Available on ORBi :
since 06 April 2009

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