Article (Scientific journals)
Light induced functionalization of PCL-PEG block copolymers for the covalent immobilization of biomolecules
Pourcelle, Vincent; Freichels, Hélène; Stoffelbach, François et al.
2009In Biomacromolecules, 10 (4), p. 966-974
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Keywords :
ring-opening polymerization (ROP); lactone; époxide
Abstract :
[en] Functionalized poly-ε-caprolactone-block-polyethyleneglycol (PCL-PEG) amphiphilic copolymers were prepared to be constituents of nanocarriers used for the targeting of specific cells. Hence, we conceived a smooth and simple photografting methodology on these copolymers using a bifunctional molecular clip (O-succinimidyl-4-(p-azido-phenyl)butanoate). We prepared PCL-PEGs with pendent N-hydroxysuccinimide esters and studied the grafting with 3H-lysine, which radioactivity was counted by LSC. Several parameters were investigated, such as behavior of homopolymers, initial concentrations, irradiation, and incubation durations. Evidences of a “PEG directed photografting” are discussed and this selectivity could be improved by a selective solvent technique. The photografting on different PCL-PEGs revealed a dependency of the rates to the crystallinity of the copolymers. Several controls by SEC, DLS, and TEM of the treated copolymers were realized. Lastly, the coupling of α-d-mannopyranoside ligand was performed, reaching amounts of 5400 nmol/g of PCL-PEG. This derivatized PCL-PEG enters in the preparation of nanocarriers used for the targeting of antigen presenting cells.
Research center :
Center for Education and Research on Macromolecules (CERM)
Disciplines :
Materials science & engineering
Chemistry
Author, co-author :
Pourcelle, Vincent;  Université catholique de Louvain (UCL) > Unité de Chimie Organique et Médicinale (CHOM)
Freichels, Hélène ;  Université de Liège - ULiège > Department of Chemistry > Center for Education and Research on Macromolecules (CERM)
Stoffelbach, François;  Université de Liège - ULiège > Department of Chemistry > Center for Education and Research on Macromolecules (CERM)
Auzély-Velty, Rachel;  Grenoble, France > Recherches sur les Macromolécules Végétales (CERMAV-CNRS)
Jérôme, Christine  ;  Université de Liège - ULiège > Department of Chemistry > Center for Education and Research on Macromolecules (CERM)
Marchand-Brynaert, Jacqueline;  Université catholique de Louvain (UCL) > Unité de Chimie Organique et Médicinale (CHOM)
Language :
English
Title :
Light induced functionalization of PCL-PEG block copolymers for the covalent immobilization of biomolecules
Publication date :
13 April 2009
Journal title :
Biomacromolecules
ISSN :
1525-7797
eISSN :
1526-4602
Publisher :
American Chemical Society, Washington, United States - District of Columbia
Volume :
10
Issue :
4
Pages :
966-974
Peer reviewed :
Peer Reviewed verified by ORBi
Name of the research project :
VACCINOR program
The "CGRI-FNRS-CNRS" cooperation program
Funders :
Walloon region [BE]
F.R.S.-FNRS - Fonds de la Recherche Scientifique [BE]
BELSPO - Service Public Fédéral de Programmation Politique scientifique [BE]
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