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Pregnancy-Associated Glycoproteins (PAGs) in prolific (Boujaâd x D’man) and non- prolific (Boujaâd) Moroccan sheep by means of four radio-immunoassays
El Amiri, Bouchra; Melo de Sousa, Noelita; El Abbadi, N et al.
2013In Proceedings of the 23rd International Congress of Hungarian Association for Buiatrics
 

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Keywords :
PAGs,; Moroccan sheep; Pregnancy diagnosis; different RIA systems
Abstract :
[en] The aim of the present study was: i) to investigate the use of PAGs for pregnancy follow up in Boujaâd non prolific (n=8) vs. Boujaâd x D’man a prolific sheep (n=20), ii) to study whether the boPAG tracer (daily available at the laboratory of physiology of reproduction, Liège, could be used in RIA without affecting the results, iii) to prospect the possibility of transferring the technique to a Moroccan laboratory. The blood samples were weekly collected from the first week of gestation till the 4 week after lambing. The RIA1, RIA2, RIA3 and RIA4 were all based on ovPAG standard, while the tracer and antiserum changed according to the system. The RIA1, RIA3 and RIA4 were performed in Belgium while the RIA2 was carried out in Morocco. The results showed that the highest correlation was obtained between the RIA3 and RIA4 (R2 = 0.83) while the lowest was recorded between the RIA2 and RIA4 (R2 = 0.69). The lowest concentrations derived from the RIA2. The highest concentrations were recorded before lambing (279.29; 88.21; 268.75; 152.67 ng/ml respectively for RIAs 1, 2, 3 and 4). To conclude, the profiles of ovPAG showed that it is possible to flow-up with success using the four systems. In addition, the RIA1 using boPAG as tracer could be a good alternative to the homologous one and finally, it is possible to set up routinely the RIA technique for PAG detection in Moroccan laboratories.
Disciplines :
Veterinary medicine & animal health
Author, co-author :
El Amiri, Bouchra;  Institut Scientifique de Recherche Agronomique - INRA > Centre Régional de Settat, Settat, Maroc
Melo de Sousa, Noelita ;  Université de Liège - ULiège > Département de sciences fonctionnelles > Physiologie de la reproduction
El Abbadi, N;  Centre National de l’Energie, des Sciences et Techniques Nucléaires Rabat, Maroc
El Fadili, M;  Institut Scientifique de Recherche Agronomique - INRA > Productions Animales, Rabat, Maroc
Szenci, Otto;  MTA-SZIE Large Animal Clinical Research Group, Üllő, Hungary
Beckers, Jean-François  ;  Université de Liège - ULiège > Département de sciences fonctionnelles > Physiologie de la reproduction
Language :
English
Title :
Pregnancy-Associated Glycoproteins (PAGs) in prolific (Boujaâd x D’man) and non- prolific (Boujaâd) Moroccan sheep by means of four radio-immunoassays
Publication date :
2013
Event name :
23rd International Congress of Hungarian Association for Buiatrics
Event organizer :
Hungarian Association for Buiatrics
Event place :
SIÓFOK, Hungary
Event date :
16-19 OCTOBER 2013
Audience :
International
Main work title :
Proceedings of the 23rd International Congress of Hungarian Association for Buiatrics
Publisher :
Hungarian Association for Buiatrics, Budapest, Hungary
Edition :
Hungarian Association for Buiatrics
Pages :
177-181
Available on ORBi :
since 30 October 2013

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