Crotalaria; biodiversity conservation; indigeous organism; seed germination; root length; in vitro experimentation; copper; soil chemicophysical prperties; D. R. Congo
Abstract :
[en] Description of the subject. Microevolutionary processes in metallophytes established on copper enriched soils can lead to a diversity of plant species showing distinct tolerance capacities among genus. Researches about the relationship between these endangered plants and soil copper concentrations are critical in order to improve ex situ conservation methods in southeastern Democratic Republic of Congo (Katangan Copperbelt). Objectives. The aim of the study was to test the effect of copper on the germination and root elongation of three Crotalariaspecies naturally occurring along a natural copper gradient. The hypothesis is that copper concentrations have different effects on germination and root elongation according to the species of Crotalaria genus.Method. Three species were selected: Crotalaria cobalticola, Crotalaria peschiana and Crotalariacornetii, occurring on soils with the highest to the lowest copper concentrations respectively. Germination and root elongation tests were performed in vitro (MS vitamin-enriched medium) in six copper mediums ranging from 0 to 125 μM Cu2+. Results. No significant differences in germination percentage were observed according to the copper concentrations. Crotalaria cornetii had the lowest germination percentage. Root elongation of C. peschiana did not differ with copper concentration, but root elongation of C. cobalticola was higher at the greatest copper concentration (125 μM Cu2+). Conclusions. Even if C. cobalticola presented better growth at highest Cu concentrations, it appeared that C. cobalticola and C. peschiana do not require copper for their early stages of development and could thus be conserved in non-contaminated substrate. Crotalariacornetii seemed to present a physical seed dormancy.
Disciplines :
Environmental sciences & ecology
Author, co-author :
Boisson, Sylvain ; Université de Liège > Ingénierie des biosystèmes (Biose) > Biodiversité et Paysage
Le Stradic, Soizig ; Université de Liège > Ingénierie des biosystèmes (Biose) > Biodiversité et Paysage
Commans, Morgane
Dumont, Amandine
Leclerc, Natasha
Thomas, Cynthia ; Université de Liège > Ingénierie des biosystèmes (Biose) > Biodiversité et Paysage
Mahy, Grégory ; Université de Liège > Ingénierie des biosystèmes (Biose) > Biodiversité et Paysage
Language :
English
Title :
Copper tolerance of three Crotalaria species from southeastern D.R. Congo at the early development stage
Publication date :
2016
Journal title :
Biotechnologie, Agronomie, Société et Environnement
ISSN :
1370-6233
eISSN :
1780-4507
Publisher :
Presses Agronomiques de Gembloux, Gembloux, Belgium
Volume :
20
Issue :
2
Pages :
151-160
Peer reviewed :
Peer Reviewed verified by ORBi
Funders :
FRIA - Fonds pour la Formation à la Recherche dans l'Industrie et dans l'Agriculture [BE]
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