Identification of specific bovine blood biomarkers with a non-targeted approach using HPLC ESI tandem mass spectrometry

[en] Animal by-products are valuable protein sources in animal nutrition. Among them are blood products and blood meal, which are used as high-quality material for their beneficial effects on growth and health. Within the framework of the feed ban relaxation, the development of complementary methods in order to refine the identification of processed animal proteins remains challenging. The aim of this study was to identify specific biomarkers that would allow the detection of bovine blood products and processed animal proteins using tandem mass spectrometry. Seventeen biomarkers were identified: nine peptides for bovine plasma powder; seven peptides for bovine haemoglobin powder, including six peptides for bovine blood meal; and one peptide for porcine blood. They were not detected in several commercial compound feed or feed materials, such as blood by-products of other animal origins, milk-derived products and fish meal. These biomarkers could be used for developing a species-specific and blood-specific detection method.

Disciplines :

Agriculture & agronomy
Veterinary medicine & animal health

Author, co-author :

Lecrenier, Marie-Caroline ; Université de Liège - ULiège > Doct. sc. vété. (Bologne)

Marbaix, Hélène

Dieu, Marc

Veys, Pascal

Saegerman, Claude ; Université de Liège > Département des maladies infectieuses et parasitaires (DMI) > Epidémiologie et analyse des risques appl. aux sc. vétér.

Raes, Martine

Baeten, Vincent

Language :

English

Title :

Identification of specific bovine blood biomarkers with a non-targeted approach using HPLC ESI tandem mass spectrometry

Publication date :

June 2016

Journal title :

Food Chemistry

ISSN :

0308-8146

eISSN :

1873-7072

Publisher :

Elsevier Science, Oxford, United Kingdom

Volume :

213

Pages :

417-424

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 06 July 2016

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64 (10 by ULiège)

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16 (3 by ULiège)

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Bibliography

Axmann, S., Adler, A., Brandstettner, A.J., Spadinger, G., Weiss, R., Strnad, I., Species identification of processed animal proteins (PAPs) in animal feed containing feed materials from animal origin. Food Additives & Contaminants: Part A, 2015, 1–10.
Baeten, V., von Holst, C., Garrido, A., Vancutsem, J., Michotte Renier, A., Dardenne, P., Detection of banned meat and bone meal in feedstuffs by near-infrared microscopic analysis of the dense sediment fraction. Analytical and Bioanalytical Chemistry 382:1 (2005), 149–157.
Boehmer, J.L., Bannerman, D.D., Shefcheck, K., Ward, J.L., Proteomic analysis of differentially expressed proteins in bovine milk during experimentally induced Escherichia coli mastitis. Journal of Dairy Science 91:11 (2008), 4206–4218.
Boix, A., Fernández Pierna, J.A., von Holst, C., Baeten, V., Validation of a near infrared microscopy method for the detection of animal products in feedingstuffs: Results of a collaborative study. Food Additives & Contaminants: Part A 29:12 (2012), 1872–1880.
Bradley, R., Bovine spongiform encephalopathy (BSE): The current situation and research. European Journal of Epidemiology 7:5 (1991), 532–544.
Buckley, M., Collins, M., Thomas-Oates, J., Wilson, J.C., Species identification by analysis of bone collagen using matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry. Rapid Communications in Mass Spectrometry 23:23 (2009), 3843–3854.
Claydon, A.J., Grundy, H.H., Charlton, A.J., Romero, M.R., Identification of novel peptides for horse meat speciation in highly processed foodstuffs. Food Additives & Contaminants: Part A 32:10 (2015), 1718–1729.
Cunsolo, V., Muccilli, V., Saletti, R., Foti, S., Mass spectrometry in food proteomics: A tutorial. Journal of Mass Spectrometry 49:9 (2014), 768–784.
D'Alessandro, A., Zolla, L., We are what we eat: Food safety and proteomics. Journal of Proteome Research 11:1 (2012), 26–36.
Dumont, V., Kerbach, S., Poms, R., Johnson, P., Mills, C., Popping, B., et al. Development of milk and egg incurred reference materials for the validation of food allergen detection methods. Quality Assurance and Safety of Crops & Foods 2:4 (2010), 208–215.
European Commission, Commission Regulation (EC) No 152/2009 of 27 January 2009 laying down the methods of sampling and analysis for the official control of feed. Official Journal of European Union L54 (2009), 1–130.
European Commission, Regulation (EC) No 1069/2009 of 21 October 2009 laying down health rules as regards animal by-products and derived products not intended for human consumption and repealing Regulation (EC) No 1774/2002 (Animal by-products Regulation). Official Journal of European Union L300 (2009), 1–33.
European Commission, Commission Regulation (EU) No 142/2011 of 25 February 2011 implementing Regulation (EC) No 1069/2009 of the European Parliament and of the Council laying down health rules as regards animal by-products and derived products not intended for human consumption and implementing Council Directive 97/78/EC as regards certain samples and items exempt from veterinary checks at the border under that Directive Text with EEA relevance. Official Journal of European Union L54 (2011), 1–254.
European Commission, Commisson regulation (EU) No 56/2013 of 16 January 2013 amending Annexes I and IV to Regulation (EC) No 999/2001 of the European Parliament and of the Council laying down rules for the prevention, control and eradication of certain transmissible spongiform encephalopathies. Official Journal of European Union L21 (2013), 3–16.
European Union, Regulation (EC) No 999/2001 of the European Parliament and of the Council of 22 May 2001 laying down rules for the prevention, control and eradication of certain transmissible spongiform encephalopathies. Official Journal of European Union L147 (2001), 1–40.
European Union, Regulation (EC) No 1774/2002 of the European Parliament and of the Council of 3 October 2002 laying down health rules concerning animal by-products not intended for human consumption. Official Journal of European Union L273 (2002), 1–95.
Fernandez Ocana, M., Neubert, H., Przyborowska, A., Parker, R., Bramley, P., Halket, J., et al. BSE control: Detection of gelatine-derived peptides in animal feed by mass spectrometry. Analyst 129:2 (2004), 111–115.
Food Standards Agency, Industry guide on edible co-products and animal by products. 2011 < https://www.food.gov.uk/sites/default/files/multimedia/pdfs/ediblecoprod.pdf> Access Date 15/10/2015.
Fumière, O., Dubois, M., Baeten, V., von Holst, C., Berben, G., Effective PCR detection of animal species in highly processed animal byproducts and compound feeds. Analytical and Bioanalytical Chemistry 385:6 (2006), 1045–1054.
Fumière, O., Marien, A., Fernández Pierna, J.A., Baeten, V., Berben, G., Development of a real-time PCR protocol for the species origin confirmation of isolated animal particles detected by NIRM. Food Additives & Contaminants: Part A 27:8 (2010), 1118–1127.
Fumière, O., Veys, P., Boix, A., von Holst, C., Baeten, V., Berben, G., Methods of detection, species identification and quantification of processed animal proteins in feedingstuffs. Biotechnologie, Agronomie, Société et Environnement (BASE) 13 (2009), 59–70.
Kats, L.J., Nelssen, J.L., Tokach, M.D., Goodband, R.D., Hansen, J.A., Laurin, J.L., The effect of spray-dried porcine plasma on growth performance in the early-weaned pig. Journal of Animal Science 72:8 (1994), 2075–2081.
Lallès, J.P., Bosi, P., Janczyk, P., Koopmans, S.J., Torrallardona, D., Impact of bioactive substances on the gastrointestinal tract and performance of weaned piglets: A review. Animal 3:12 (2009), 1625–1643.
Lecrenier, M.C., Ledoux, Q., Berben, G., Fumière, O., Saegerman, C., Baeten, V., et al. Determination of the ruminant origin of bone particles using fluorescence in situ hybridization (FISH). Scientific Reports, 4, 2014.
Liu, X., Han, L., Veys, P., Baeten, V., Jiang, X., Dardenne, P., An overview of the legislation and light microscopy for detection of processed animal proteins in feeds. Microscopy Research and Technique 74:8 (2011), 735–743.
Marbaix, H., Budinger, D., Dieu, M., Fumière, O., Gillard, N., Delahaut, P., et al. Identification of proteins and peptide biomarkers for detecting banned Processed Animal Proteins (PAPs) in meat and bone meal by mass spectrometry. Journal of Agricultural and Food Chemistry 64:11 (2016), 2405–2414.
Myers, M.J., Yancy, H.F., Farrell, D.E., Washington, J.D., Deaver, C.M., Frobish, R.A., Assessment of two enzyme-linked immunosorbent assay tests marketed for detection of ruminant proteins in finished feed. Journal of Food Protection 70:3 (2007), 692–699.
Nesvizhskii, A.I., Keller, A., Kolker, E., Aebersold, R., A statistical model for identifying proteins by tandem mass spectrometry. Analytical Chemistry 75:17 (2003), 4646–4658.
Ofori, J.A., Hsieh, Y.H., Sandwich enzyme-linked immunosorbent assay for the detection of bovine blood in animal feed. Journal of Agricultural and Food Chemistry 55:15 (2007), 5919–5924.
Piras, C., Roncada, P., Rodrigues, P.M., Bonizzi, L., Soggiu, A., Proteomics in food: Quality, safety, microbes and allergens. Proteomics, 2015.
Plouvier, B.M., Baeten, V., Maudoux, J.P., Vanopdenbosch, E., Berkvens, D., Degand, G., et al. Detection of processed animal protein: European experience and perspectives. Revue Scientifique et Technique 31:3 (2012), 1011–1031.
Reece, P., Chassaigne, H., Collins, M., Buckley, M., Bremer, M., Grundy, H.H., Proteomic analysis of meat and bone meal and animal feed. Jorgensen, J.S., Baeten, V., (eds.) Detection, identification and quantification of processed animal proteins in feedingstuffs, 2012, Presses universitaires de Namur, Namur, 113–124.
Sentandreu, M.A., Sentandreu, E., Peptide biomarkers as a way to determine meat authenticity. Meat Science 89:3 (2011), 280–285.
Swaisgood, H.E., Protein and Amino Acid Composition of Bovine Milk. Jensen, R.G., (eds.) Handbook of milk composition, 1995, Academic Press, San Diego, 464–468.
van Raamsdonk, L.W.D., Margry, R.J.C.F., Van Kaathoven, R.G.C., Bremer, M.G.E.G., Inter-laboratory validation study of two immunochemical methods for detection of processed ruminant proteins. Food Chemistry 185 (2015), 333–339.
van Raamsdonk, L.W.D., Pinotti, L., Veys, P., Bremer, M., Hekman, W., Kemmers, A., et al. New developments in classical microscopy; what can be expected for the official control?. Biotechnologie, Agronomie, Société et Environnement 15 (2011), 15–24.
Veys, P., Berben, G., Baeten, V., CRL-AP proficiency test 2009. 2010 < http://www.eurl.craw.eu/img/page/proficiency/CRL_AP_PT_2009_Final_report.pdf> Access Date 24/11/2015.
Veys, P., Berben, G., Baeten, V., EURL-AP proficiency test microscopy 2014. 2015 < http://eurl.craw.eu/img/page/proficiency/EURL-AP%20report%202014%20final.pdf> Access Date 15/12/2015.
Veys, P., Berben, G., Dardenne, P., Baeten, V., Detection and identification of animal by-products in animal feed for the control of transmissible spongiform encephalopathies. Fink-Gremmels, J., (eds.) Animal feed contamination—Effects on livestock and food safety, 2012, Woodhead Publishing Ltd, Cambridge, 104–110.
Yang, Z., Han, L., Fernández Pierna, J.A., Dardenne, P., Baeten, V., Review: The potential of near infrared microscopy to detect, identify and quantify processed animal by-products. Journal of Near Infrared Spectroscopy 19:4 (2011), 211–231.