Effect Of Imbalance Between Energy And Nitrogen Supplies On Microbial Protein Synthesis And Nitrogen Metabolism In Growing Double-Muscled Belgian Blue Bulls
[en] Six double-muscled Belgian Blue bulls (initial weight: 345 ± 16 kg) with cannulas in the rumen
and proximal duodenum were used in two juxtaposed 3 × 3 Latin squares to study the effect of a lack of synchronization between energy and N in the rumen on microbial protein synthesis and N metabolism by giving the same diet according to three different feeding patterns. The feed ingredients of the diet were separated into two groups supplying the same amount of
fermentable OM (FOM), but characterized by different levels of ruminally degradableN (RDN). The first group primarily provided energy for the ruminal microbes (14.6 g of RDN/kg of FOM), and the second provided N (33.3 g of RDN/kg of FOM). These two groups were fed to the bulls simultaneously or alternately with the aim of creating three different time periods of imbalance (0, 12, or 24 h) between energy and N supplies in the rumen. The introduction of imbalance affected neither microbial-N flow at the duodenum (P = 0.65) nor efficiency of growth (P = 0.69), but decreased (P = 0.016) the NDF degradation in the rumen 12.2% for a 12-h period of imbalance. N retention was not affected by imbalance (P = 0.53) and reached 57.8, 58.5, and 54.7 g/d, respectively, for 0-, 12- and 24-h imbalance. It seems that the introduction of an imbalance of 12 or 24 h between energy and N supplies for the ruminal microbes by altering the feeding pattern of the same diet does not negatively influence microbial protein synthesis
or N retention by the animal. Nitrogen recycling in the rumen plays a major role in regulating the amount of ruminally availableNand allows for continuous synchronization of N- and energy-yielding substrates for the microorganisms in the rumen. Therefore, a lack of synchronization in the diet between the energy and N supplies for the ruminal microbes is not detrimental to their growth or for the animal as long as the nutrient supply is balanced on a 48-h basis. Thus, these dietary feeding patterns may be used under practical feeding conditions with minimal effect on the performance of ruminant animals.
Effect Of Imbalance Between Energy And Nitrogen Supplies On Microbial Protein Synthesis And Nitrogen Metabolism In Growing Double-Muscled Belgian Blue Bulls
Publication date :
2004
Journal title :
Journal of Animal Science
ISSN :
0021-8812
eISSN :
1525-3163
Publisher :
American Society of Animal Science, Savoy, United States - Illinois
AOAC. 1990. Official Methods of Analysis. 15th ed. Assoc. Offic. Anal. Chem., Arlington, VA.
Beckers, Y., A. Théwis, C. Sohy, and E. François. 1995. Measurements of microbial N flow to the duodenum and urinary excretion of purine derivatives in bulls. Ann. Zootech. 44(Suppl.):177.
Casper, D. P., H. A. Maiga, M. J. Brouk, and D. J. Schingoethe. 1999. Synchronization of carbohydrate and protein sources on fermentation and passage rates in dairy cows. J. Dairy Sci. 82:1779-1790.
CVB. 2000. Veevoedertabel. Centraal Veevoederbureau, Lelystad, The Netherlands.
Czerwaski, J. M. 1986. An Introduction to Rumen Studies. Pergammon Press, Oxford, U.K.
Dagnelie, P. 1986. Théories et Méthodes Statistiques. Vol 2. Presses Agronomiques de Gembloux, Belgium.
Dawson, J. M. 1999. Variation in nutrient supply and effects on whole body anabolism. Pages 101-126 in Proc. VIIIth Int. Symp. on Protein Metab. and Nutr. G. E. Lobley, A. White, J. C. MacRae, ed. EAAP Publications, Wageningen, The Netherlands.
De Campeneere, S. 2000. Energy and protein standards for finishing Belgian Blue double-muscled bulls. Ph.D. Diss., Faculteit landbouwkundige en toegepaste biologishe wetenschappen, Ghent Univ., Ghent, Belgium.
Dehareng, D., and B. B. Ndibualonji. 1994. Naissance et destinée de l'ammoniaque ruminale: Revue. 2. Absorption et destinée ultérieure. Empoisonnement à l'ammoniaque. Ann. Méd. Vét. 138:387-398.
Dewhurst, R. J., D. R. Davies, and R. J. Merry. 2000. Microbial protein supply from the rumen. Anim. Feed Sci. Technol. 85:1-21.
Fiems, L. O., B. G. Cottyn, Ch. V. Boucqué, D. F. Bogaerts, C. Van Eenaeme, and J. M. Vanacker. 1997. Effect of beef type, body weight and dietary protein content on voluntary feed intake, digestibility, blood and urine metabolites and nitrogen retention. J. Anim. Physiol. A. Anim. Nutr. 77:1-9.
François, E., N. Thill, and A. Théwis. 1978. Méthode rapide de dosage de l'oxyde de chrome dans les aliments, les fèces et les contenus digestifs par titrage après oxydation nitroperchlorique. Ann. Zootech. 27:355-361.
Froidmont, E., Y. Beckers, and A. Théwis. 2000. Determination of the methionine requirement of growing double-muscled Belgian Blue bulls with a three-step method. J. Anim. Sci. 82:233-241.
Gustafsson, A. H., and D. L. Palmquist. 1993. Diurnal variation of rumen ammonia, serum urea and milk urea in dairy cows at high and low yields. J. Dairy Sci. 76:475-484.
Henning, P. H., D. G. Steyn, and H. H. Meissner. 1993. Effect of synchronization of energy and nitrogen supply on ruminal characteristics and microbial growth. J. Anim. Sci. 71:2516-2528.
Hornick, J. L., C. Van Eenaeme, A. Clinquart, M. Diez, and L. Istasse. 1998. Different periods of feed restriction before compensatory growth in Belgian Blue bulls: I. Animal performance, nitrogen balance, meat characteristics, and fat composition. J. Anim. Sci. 76:249-259.
Huntington, G. B., and S. L. Archibeque. 2000. Practical aspects of urea and ammonia metabolism in ruminants. Proc. Am. Soc. Anim. Sci. 1999. Available: http://www.asas.org/jas/symposia/proceedings/0939.pdf. Accessed Dec. 15, 2000.
Kim, K. H., J. J. Choung, and D. G. Chamberlain. 1999a. Effect of varying the degree of synchrony of energy and nitrogen release in the rumen on the synthesis of microbial protein in lactating dairy cows consuming a diet of grass silage and a cereal-based concentrate. J. Sci. Food Agric. 79:1441-1447.
Kim, K. H., Y. G. Oh, J. J. Choung, and D. G. Chamberlain. 1999b. Effects of varying degrees of synchrony of energy and nitrogen release in the rumen on the synthesis of microbial protein in cattle consuming grass silage. J. Sci. Food Agric. 79:833-838.
Ludden, P. A., T. L. Wechter, and B. W. Hess. 2002. Effects of oscillating dietary protein on ruminal fermentation and site and extent of nutrient digestion in sheep. J. Anim. Sci. 80:3336-3346.
Kim, E. J., D. S. Parker, and N. D. Scollan. 2001. Fishmeal supplementation of steers fed on grass silage: Effects on rumen function, nutrient flow to and disappearance from the small intestine. Anim. Res. 50:337-348.
Makkar, H. P. S., and K. Becker. 1999. Technical report: Purine quantification in digesta from ruminants by spectrometric and HPLC methods. J. Nutr. 81:107-112.
Milton, C. T., R. T. Brandt, Jr., and E. C. Titgemeyer. 1997. Urea in dry-rolled corn diets: Finishing steer performance, nutrient digestion and microbial protein production. J. Anim. Sci. 75:1415-1424.
Newbold, J. R., and S. R. Rust. 1992. Effect of asynchronous nitrogen and energy supply on growth of ruminal bacteria in batch culture. J. Anim. Sci. 70:538-546.
Obispo, N. E., and B. A. Dehority. 1999. Feasibility of using total purines as a marker for ruminal bacteria. J. Anim. Sci. 77:3084-3095.
Poncet, C., and D. Rémond. 2002. Rumen digestion and intestinal nutrient flows in sheep consuming pea seeds: The effect of extrusion or chesnut tannin addition. Anim. Res. 51:201-216.
Richardson, J. M., R. G. Wilkinson, and L. A. Sinclair. 2003. Synchrony of nutrient supply to the rumen and dietary energy source and their effects on the growth and metabolism of lambs. J. Anim. Sci. 81:1332-1347.
Satter, L. D., and L. L. Slyter. 1974. Effect of ammonia concentration on rumen microbial protein production in vitro. Br. J. Nutr. 32:199-208.
Sauvant, D., and J. van Milgen. 1995. Dynamic aspects of carbohydrate and protein breakdown and the associated microbial matter synthesis. Pages 71-91 in Proc. 8th Int. Symp. Rumin. Physiol. W. V. Engelhardt, S. Leonhard-Marek, G. Breves, and D. Giesecke, ed. Ferdinand Enke Verlag, Stuttgart, Germany.
Tamminga, S., W. N. Van Straalen, A. P. J. Subnel, R. G. M. Meijer, A. Steg, C. J. G. Wener, and M. C. Block. 1994. The Dutch protein evaluation system: The DVE/OEB system. Livest. Prod. Sci. 40:139-155.
Van Es, A. J. H., and Y. Van der Honing. 1977. Het nieuwe energetische voederwaarderingssyteem voor herkauwers: Wijze van afleiding en uiteindelijk voorstel. Report IVVO. 92:1-48.
Van Soest, P. J., J. B. Robertson, and B. A. Lewis. 1991. Methods for dietary fiber, neutral-detergent fiber and non-starch polysaccharides in relation to animal nutrition. J. Dairy Sci. 74:3583-3597.
Veenhuizen, J. J., A. D. Mc Gilliard, and J. W. Young. 1984. Apparatus for total collection of urine from steers. J. Dairy Sci. 67:1865-1867.
Witt, M. W., L. A. Sinclair, R. G. Wilkinson, and P. J. Buttery. 1999a. The effects of synchronizing the rate of dietary energy and nitrogen supply to the rumen on the production and metabolism of sheep: Food characterization and growth and metabolism of ewe lambs given food ad libitum. Anim. Sci. 69:223-235.
Witt, M. W., L. A. Sinclair, R. G. Wilkinson, and P. J. Buttery. 1999b. The effects of synchronizing the rate of dietary energy and nitrogen supply to the rumen on the metabolism and growth of ram lambs given food at a restricted level. Anim. Sci. 69:627-636.
Zinn, R. A., and F. N. Owens. 1986. A rapid procedure for purine measurement and its use for estimating net ruminal protein synthesis. Can. J. Anim. Sci. 66:157-166.