[en] To test the hypothesis that CD95-L (Fas-L) present on trophoblastic cells plays a part in establishing foeto-placental tolerance by inducing apoptosis of immune defence cells, we cocultured trophoblasts with lymphoid cells and scored the frequency of cell death in these cultures. We prepared human trophoblastic cells from term placentas removed by C-section and placed them in culture for 48 h before introducing the lymphoid cells. We added Jurkat cells, a CD3 + lymphoid cell line, or purified T cells from human blood to the cultured trophoblasts and monitored apoptosis by electron microscopy and flow cytometry after TUNEL or annexin V labelling. The frequency of cell death in the CD3 + cell population was higher when the lymphoid cells were cocultured with trophoblastic cells than when they were cultured alone. This frequency increased with time but was reduced when anti-CD95-L antibodies were added to the culture medium. Cell death was less frequent in the lymphoid cell population when trophoblasts were replaced with human fibroblasts not expressing CD95-L. (C) 1999 Elsevier Science B.V. All rights reserved.
Coumans, Bernard ; Université de Liège - ULiège > CNCM/ Centre fac. de rech. en neurobiologie cell. et moléc.
Thellin, Olivier ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Histologie humaine
Zorzi, Willy ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Histologie humaine
Mélot, France ; Université de Liège - ULiège > Services généraux (Faculté de médecine) > Service administratif de la Faculté (Médecine)
Bougoussa, M.
Melen-Lamalle, Laurence ; Université de Liège - ULiège > Département des sciences cliniques > Labo de biologie des tumeurs et du développement
Zorzi, Danièle ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Département des sciences biomédicales et précliniques
Hennen, Georges ; Centre Hospitalier Universitaire de Liège - CHU > Endocrinologie clinique
Igout, Ahmed ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Département des sciences biomédicales et précliniques
Heinen, Ernst ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Histologie humaine
Language :
English
Title :
Lymphoid cell apoptosis induced by trophoblastic cells: a model of active foeto-placental tolerance
Akcali K.C., Khan S.A., Moulton B.C. Effect of decidualization on the expression of bax and bcl-2 in rat uterine endometrium. Endocrinology. 137:1996;3123-3131.
Bouzahzah Human germinal center CD4+CD57+T cells act differently on B cells than do classical T-helper cells. Dev. Immunol. 4:1995;189-197.
Bramberger A.M., Schulte H.M., Thuneke I., Erdmann I., Bramberger C.M., ASA S.L. Expression of the apoptosis-inducing Fas-Ligand (Fas-L) in human first and third trimester placenta and choriocarcinoma cells. J. Clin. Endocrinol. Metab. 82:1997;3173-3175.
Chaouat G., Menu E., Delage G., Moreau J.F., Hui L., Meliani A.A., Martal J., Raghupathy R., Lelaidier C.et al. Immuno-endocrine interactions in early pregnancy. Hum. Reprod. 10:1995;55-59. Suppl. 2.
Douglas G.C., King B.F. Isolation of the pure villous cytotrophoblast from term human placenta using immunomagnetic microspheres. J. Immunol. Methods. 119:1989;259-268.
French L.E., Hahne M., Viard I., Radlgruber G., Zanone R., Becker K., Müller C., Tschopp J. Fas and Fas Ligand in embryos and adult mice: ligand expression in several immune-privileged tissues and coexpression in adult tissues characterized by apoptotic cell turnover. J. Cell Biol. 133:1996;335-343.
Gotlieb W.H. Immunologie de la grossesse. Rev. Med. Brux. 13:1992;97-101.
Hunt J.S., Vassmer D., Ferguson T.A., Miller L. Fas ligand is positioned in mouse uterus and placental to prevent trafficking of activated leukocytes between the mother and the conceptus. J. Immunol. 158:1997;4122-4128.
Kliman H.K., Nestler J.E., Sermasi E., Sanger J.M., Strauss III J.F. Purification, characterization and in vitro differentiation of cytotrophoblasts from human term placentae. Endocrinology. 118(4):1986;1567-1582.
Letellier C., Fizet D., Baltz T., Vezon G. Immunosuppressive activity of sera of pregnant women on cytotoxic T-lymphocyte-mediated cytolysis: I. Characterization of the active fraction. Gynecol. Obstet. Invest. 37:1994;1-5.
Runic R., Lockwood C.J., Ma Y., Dipasquale B., Guller S. Expression of Fas ligand by human cytotrophoblasts: implications in placentation and fetal survival. J. Clin. Endocrinol. Metab. 81:1996;3119-3122.
Smith S.C., Baker P.N., Symonds E.M. Placental apoptosis in normal human pregnancy. Am. J. Obstet. Gynecol. 177:1997;57-65.
Uckan D., Steele A., Cherry B.Y., Chamizo W., Koutsonikolis A., Gilbert-Barness E., Good R.A. Trophoblasts express Fas ligand: a proposed mechanism for immune privilege in placenta and maternal invasion. Mol. Hum. Reprod. 3:1997;655-662.
Xerri L., Devillard E., Hassoun J., Mawas C., Birg F. Fas ligand is not only expressed in immune privileged human organs but is also coexpressed with Fas in various epithelial tissues. J. Clin. Pathol. 50:1997;87-91.
Zorzi W., Thellin O., Coumans B., Melot F., Hennen G., Lakaye A., Igout A., Heinen E. Demonstration of the expression of CD95 ligand transcript an protein in humen placenta. Placenta. 19:1998;269-277.