Ultra-high-performance liquid chromatography-mass spectrometry method for neutrophil gelatinase-associated lipocalin as a predictive biomarker in acute kidney injury

Ion, Valentin; Nys, Gwenaël; COBRAIVILLE, Gaël; Cavalier, Etienne; Crommen, Jacques; Servais, Anne-Catherine; Muntean, D.-L.; Fillet, Marianne

doi:10.1016/j.talanta.2018.11.050

Article (Scientific journals)

Ultra-high-performance liquid chromatography-mass spectrometry method for neutrophil gelatinase-associated lipocalin as a predictive biomarker in acute kidney injury

Ion, Valentin; Nys, Gwenaël; COBRAIVILLE, Gaël et al.

2019 • In Talanta, 195, p. 668-675

Peer Reviewed verified by ORBi

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https://hdl.handle.net/2268/232657

DOI
10.1016/j.talanta.2018.11.050

PubMed
30625599

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Keywords :

AKI; NGAL; Targeted proteomics; UHPLC-MS/MS; Body fluids; High performance liquid chromatography; Mass spectrometry; Molecular biology; Phase separation; Human plasma samples; Internal standards; Neutrophil gelatinase-associated lipocalin; Solid-phase extraction; U-HPLC-MS; Ultra high performance liquid chromatography-mass spectrometries; Proteins

Abstract :

[en] Neutrophil gelatinase associated lipocalin (NGAL) is a protein that was found to be overexpressed in acute kidney injury (AKI). The rise in NGAL concentration, both in urine or plasma, appears earlier than for other classical renal function markers such as serum creatinine, thus making it a suitable marker for early pathology detection. The aim of this study was to develop a method involving tryptic digestion, solid phase extraction and LC-MS/MS analysis to analyze NGAL in plasma medium using an isotope labeled surrogate protein, containing NGAL signature tags, as internal standard (QPrEST). The method was validated for the analysis of NGAL in an analytical range from 50 to 1250 ng/mL using two different proteotypic peptides. The method was further used to quantify the NGAL in human plasma samples for whom elevated NGAL values were expected. NGAL values were between 190.8 and 242.6 ng/mL for control group and between 228.1 and 3526.2 ng/mL for patient group. This study proved that the selection of the right internal standard is of utmost importance in targeted proteomics studies as the digestion steps might cause high variability. This study also confirmed that, although NGAL is highly resistant to proteases such as trypsin, the method could be fully validated according to FDA guidelines and subsequently used to assess NGAL levels in patient plasma with high analytical confidence. © 2018

Research center :

CIRM - Centre Interdisciplinaire de Recherche sur le Médicament - ULiège

Disciplines :

Pharmacy, pharmacology & toxicology

Author, co-author :

Ion, Valentin ; Université de Liège - ULiège > Doct. sc. bioméd. & pharma. (paysage)

Nys, Gwenaël ; Université de Liège - ULiège > Département de pharmacie > Analyse des médicaments

COBRAIVILLE, Gaël ; Université de Liège - ULiège > Département de pharmacie > Analyse des médicaments

Cavalier, Etienne ; Université de Liège - ULiège > Département de pharmacie > Chimie médicale

Crommen, Jacques ; Université de Liège - ULiège > Département de pharmacie > Département de pharmacie

Servais, Anne-Catherine ; Université de Liège - ULiège > Département de pharmacie > Analyse des médicaments

Muntean, D.-L.; Analytical Chemistry and Drug Analysis Department, Faculty of Pharmacy, University of Medicine, Pharmacy, Sciences and Technology from Tîrgu Mureș, Tîrgu Mureș, 540139, Romania

Fillet, Marianne ; Université de Liège - ULiège > Département de pharmacie > Analyse des médicaments

Language :

English

Title :

Ultra-high-performance liquid chromatography-mass spectrometry method for neutrophil gelatinase-associated lipocalin as a predictive biomarker in acute kidney injury

Publication date :

2019

Journal title :

Talanta

ISSN :

0039-9140

eISSN :

1873-3573

Publisher :

Elsevier B.V.

Volume :

195

Pages :

668-675

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 07 February 2019

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