[en] Single-cell mRNA-sequencing (scRNA-seq) is a technique which enables unbiased, high throughput and high-resolution transcriptomic analysis of the heterogeneity of cells within a population. This recent technique has been described in humans, mice and other species in various conditions to cluster cells in populations and identify new subpopulations, as well as to study the gene expression of cells in various tissues, conditions and origins. In dogs, a species for which markers of cell populations are often limiting, scRNA-seq presents with elevated yet untested potential for the study of tissue composition. As a proof of principle, we used scRNA-seq to identify cellular populations of the bronchoalveolar lavage fluid (BALF) in healthy dogs (n = 4). A total of 5710 cells were obtained and analyzed by scRNA-seq. Fourteen distinct clusters of cells were identified, further identified as macrophages/monocytes (4 clusters), T cells (2 clusters) and B cells (1 cluster), neutrophils (1 cluster), mast cells (1 cluster), mature or immature dendritic cells (1 cluster each), ciliated or non-ciliated epithelial cells (1 cluster each) and cycling cells (1 cluster). We used for the first time in dogs the scRNA-seq to investigate cellular subpopulations of the BALF of dog. This study hence expands our knowledge on dog lung immune cell populations, paves the way for the investigation at single-cell level of lower respiratory diseases in dogs, and establishes that scRNA-seq is a powerful tool for the study of dog tissue composition.
Disciplines :
Life sciences: Multidisciplinary, general & others Veterinary medicine & animal health Immunology & infectious disease Biotechnology
Author, co-author :
Fastrès, Aline ; Université de Liège - ULiège > Dép. clinique des animaux de compagnie et des équidés (DCA) > Médecine interne des animaux de compagnie
Pirottin, Dimitri ; Université de Liège - ULiège > Département des sciences fonctionnelles (DSF) > Département des sciences fonctionnelles (DSF)
Fievez, Laurence ; Université de Liège - ULiège > I3-Cellular and Molecular Immunology
Marichal, Thomas ; Université de Liège - ULiège > I3-Immunophysiology
Desmet, Christophe ; Université de Liège - ULiège > I3-Cellular and Molecular Immunology
Bureau, Fabrice ; Université de Liège - ULiège > Vice-Recteur à la Recherche
Clercx, Cécile ; Université de Liège - ULiège > Dép. clinique des animaux de compagnie et des équidés (DCA) > Pathologie médicale des petits animaux
Language :
English
Title :
Characterization of the bronchoalveolar lavage fluid by single cell gene expression analysis in healthy dogs: a promising technique.
Publication date :
30 July 2020
Journal title :
Frontiers in Immunology
eISSN :
1664-3224
Publisher :
Frontiers Media S.A., Switzerland
Volume :
11
Pages :
1707
Peer reviewed :
Peer Reviewed verified by ORBi
Funders :
ULg FSR - Université de Liège. Fonds spéciaux pour la recherche [BE]
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