bta-miR-23a Regulates the Myogenic Differentiation of Fetal Bovine Skeletal Muscle-Derived Progenitor Cells by Targeting MDFIC Gene

Hu, Xin; Xing, Yishen; Ren, Ling; Wang, Yahui; Li, Qian; Yang, Qiyuan; Du, Min; Xu, Lingyang; Willems, Luc; Li, Junya; Zhang, Lupei

doi:10.3390/genes11101232

Article (Scientific journals)

bta-miR-23a Regulates the Myogenic Differentiation of Fetal Bovine Skeletal Muscle-Derived Progenitor Cells by Targeting MDFIC Gene

Hu, Xin; Xing, Yishen; Ren, Ling et al.

2020 • In Genes, 20, p. 1232

Peer Reviewed verified by ORBi

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https://hdl.handle.net/2268/252976

DOI
10.3390/genes11101232

PubMed
33092227

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Keywords :

bta-miR-23a; fetal muscle development; MDFIC

Abstract :

[en] miR-23a, a member of the miR-23a/24-2/27a cluster, has been demonstrated to play pivotal roles in many cellular activities. However, the mechanisms of how bta-miR-23a controls the myogenic differentiation (MD) of PDGFRα − bovine progenitor cells (bPCs) remain poorly understood. In the present work, bta-miR-23a expression was increased during the MD of PDGFRα− bPCs. Moreover, bta-miR-23a overexpression significantly promoted the MD of PDGFRα− bPCs. Luciferase reporter assays showed that the 3’-UTR region of MDFIC (MyoD family inhibitor domain containing) could be a promising target of bta-miR-23a, which resulted in its post-transcriptional down-regulation. Additionally, the knockdown of MDFIC by siRNA facilitated the MD of PDGFRα− bPCs, while the overexpression of MDFIC inhibited the activating effect of bta-miR-23a during MD. Of note, MDFIC might function through the interaction between MyoG transcription factor and MEF2C promoter. This study reveals that bta-miR-23a can promote the MD of PDGFRα− bPCs through post-transcriptional downregulation of MDFIC.

Disciplines :

Veterinary medicine & animal health

Author, co-author :

Hu, Xin

Xing, Yishen

Ren, Ling

Wang, Yahui

Li, Qian

Yang, Qiyuan

Du, Min

Xu, Lingyang

Willems, Luc ; Université de Liège - ULiège > Cancer-Cellular and Molecular Epigenetics

Li, Junya

Zhang, Lupei

Language :

English

Title :

bta-miR-23a Regulates the Myogenic Differentiation of Fetal Bovine Skeletal Muscle-Derived Progenitor Cells by Targeting MDFIC Gene

Publication date :

20 October 2020

Journal title :

Genes

ISSN :

2073-4425

Publisher :

Multidisciplinary Digital Publishing Institute (MDPI), Switzerland

Volume :

Pages :

1232

Peer reviewed :

Peer Reviewed verified by ORBi

Funders :

NSCF - National Natural Science Foundation of China [CN]
Cattle Breeding Innovative Research Team
Project of College Innovation Improvement under Beijing Municipality

Available on ORBi :

since 23 November 2020

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