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Enzyme Immobilization on Ordered Mesoporous Materials
Belet, Artium; Dondelinger, Mathieu; Gommes, Cédric et al.
2019XX International Sol-Gel Conference
 

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Abstract :
[en] In the last decades, concerns about the hazards of carcinogenic compounds found in indoor air have risen. Among those compounds, formaldehyde, which is well-known to be found in paints, tobacco smoke, open fireplaces, insulating foams, lacquers, glues, varnishes, paper and household products, is of grave concern. With the aim of removing formaldehyde from indoor air, our work focused on the immobilization of an enzyme (FDH) catalyzing the following reaction in the presence of NAD+ as an enzyme cofactor: H2CO + H2O + NAD+ + FDH ⇌ HCOOH + H+ + NADH + FDH One of the main challenges in this work was to preserve the activity of an enzyme immobilized in a harsh environment and to maintain its activity for a long time. Ordered mesoporous silica and periodic mesoporous organosilicates materials have shown to be good candidates. They offer a large specific surface area and their physico-chemical properties as well as their structural properties can be tailored during (or after) their sol-gel syntheses [1,2]. FDH was produced and protein-engineered to have a His-Tag sequence, allowing purification on a Ni2+ chromatography column. Pure FDH was successfully immobilized on different supports and its activity was assessed by UV-Vis spectrometry of NADH. The immobilization was characterized by solid state 29Si NMR and by IR spectroscopy while enzyme loading was characterized by TG-DSC. The geometrical properties of supports were characterized by Hg porosimetry, nitrogen adsorption/desorption, TEM (cf. Figure 2) and by small-angle X-ray scattering. Altogether, these results allowed us to investigate relationships between the activity of immobilized enzymes and the environment provided by their supports.
Disciplines :
Chemistry
Materials science & engineering
Author, co-author :
Belet, Artium ;  Université de Liège - ULiège > Department of Chemical Engineering > Génie chimique - Nanomatériaux et interfaces
Dondelinger, Mathieu;  Université de Liège - ULiège > Department of Life Sciences > InBios Research Unit–Center for Protein Engineering
Gommes, Cédric  ;  Université de Liège - ULiège > Department of Chemical Engineering > Department of Chemical Engineering
Galleni, Moreno ;  Université de Liège - ULiège > Département des sciences de la vie > Macromolécules biologiques
Heinrichs, Benoît ;  Université de Liège - ULiège > Department of Chemical Engineering > Génie chimique - Nanomatériaux et interfaces
Language :
English
Title :
Enzyme Immobilization on Ordered Mesoporous Materials
Publication date :
2019
Event name :
XX International Sol-Gel Conference
Event place :
Saint- Petersbourg, Russia
Event date :
25-30 August 2019
Audience :
International
References of the abstract :
Book of abstracts p. 157
Available on ORBi :
since 29 November 2020

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