[en] Premise of the study: Nuclear microsatellites (nSSRs) were designed for Guibourtia tessmannii (Fabaceae, Caesalpinioideae), a
highly exploited African timber tree, to study population genetic structure and gene flow.
• Methods and Results: We developed 16 polymorphic nSSRs from a genomic library tested in three populations of G. tessmannii
and two populations of G. coleosperma. These nSSRs display three to 14 alleles per locus (mean 8.94) in G. tessmannii. Crossamplification
tests in nine congeneric species demonstrated that the genus Guibourtia contains diploid and polyploid species.
Flow cytometry results combined with nSSR profiles suggest that G. tessmannii is octoploid.
• Conclusions: nSSRs revealed that African Guibourtia species include both diploid and polyploid species. These markers will
provide information on the mating system, patterns of gene flow, and genetic structure of African Guibourtia species.
Disciplines :
Genetics & genetic processes
Author, co-author :
Tosso, Dji-ndé Félicien ; Université de Liège > Ingénierie des biosystèmes (Biose) > Laboratoire de Foresterie des régions trop. et subtropicales
Doucet, Jean-Louis ; Université de Liège > Ingénierie des biosystèmes (Biose) > Laboratoire de Foresterie des régions trop. et subtropicales
Kaymak, Esra
Daïnou, Kasso ; Université de Liège > Ingénierie des biosystèmes (Biose) > Laboratoire de Foresterie des régions trop. et subtropicales
Duminil, Jerome
Hardy, Olivier
Language :
English
Title :
Microsatellite development for the genus Guibourtia (Fabaceae, Caesalpinioideae) reveals diploid and polyploid species
Publication date :
July 2016
Journal title :
Applications in Plant Sciences
eISSN :
2168-0450
Publisher :
Botanical Society of America, St. Louis, United States - Missouri
Volume :
4
Peer reviewed :
Peer Reviewed verified by ORBi
Name of the research project :
Etude génétique et physiologiques du genre Guibourtia dans un contexte de changement climatique
Funders :
F.R.S.-FNRS - Fonds de la Recherche Scientifique [BE]
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