Bio-specific Au/Fe3+ porous spongy nanoclusters for sensitive SERS detection of Escherichia coli O157:H7

[en] Abstract: For sensitive and fast detection of Escherichia coli O157:H7, organic and inorganic hybrid Au/Fe3+ nanoclusters (NCs) were synthesized for the first time using gold nanoparticles (GNPs), bovine serum albumin, ferric chloride, phosphate-buffered saline, and antibodies. The Au/Fe3+ porous spongy NCs with large surface area showed excellent bio-specific capability for E. coli O157:H7. GNPs in Au/Fe3+ NCs functioned as signal enhancers, significantly increasing the Raman signal via the metathesis reaction product of Prussian blue and obviously improving the detection sensitivity. We combined the novel Au/Fe3+ NCs with antibody-modified magnetic nanoparticles to create a biosensor capable of sensitive detection of E. coli O157:H7, which showed a good linear response (101 to 106 cfu/mL), high detection sensitivity (2 cfu/mL), and good recovery rate (93.60–97.50%) in spiked food samples. These results make the biosensor well-suited for food safety monitoring. This strategy achieves the goal of sensitive and quantitative detection of E. coli O157:H7.

Disciplines :

Chemistry
Microbiology

Author, co-author :

Li, Yuzhi

Gao, Fei

Lu, Chang

Fauconnier, Marie-Laure ; Université de Liège - ULiège > Département GxABT > Chimie des agro-biosystèmes

Zheng, Jinkai

Language :

English

Title :

Bio-specific Au/Fe3+ porous spongy nanoclusters for sensitive SERS detection of Escherichia coli O157:H7

Publication date :

25 September 2021

Journal title :

Biosensors

eISSN :

2079-6374

Publisher :

MDPI, Basel, Switzerland

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 24 September 2021

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