Article (Scientific journals)
Proteins involved in the endoplasmic reticulum stress are modulated in synovitis of osteoarthritis, chronic pyrophosphate arthropathy and rheumatoid arthritis, and correlate with the histological inflammatory score
de Seny, Dominique; Bianchi, Elettra; Baiwir, Dominique et al.
2020In Scientific Reports, 10 (1)
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Abstract :
[en] It is now well recognized that osteoarthritis (OA) synovial membrane presents inflammatory components. The aim of this work is to provide evidence that similar inflammatory mechanisms exist in synovial membrane (n = 24) obtained from three pathologies presenting altogether an inflammatory gradient: OA, chronic pyrophosphate arthropathy (CPPA) and rheumatoid arthritis (RA). Synovial biopsies were first characterized by a histological score based on synovial hyperplasia and infiltration of lymphocytes, plasma cells, polymorphonuclear and macrophages. All biopsies were also analyzed by 2D-nano-UPLC-ESI-Q-Orbitrap for protein identification and quantification. Protein levels were correlated with the histological score. Histological score was in the range of 3 to 8 for OA, 5 to 13 for CPPA and 12 to 17 for RA. Of the 4,336 proteins identified by mass spectrometry, 51 proteins were selected for their strong correlation (p < 0.001) with the histological score of which 11 proteins (DNAJB11, CALR, ERP29, GANAB, HSP90B1, HSPA1A, HSPA5, HYOU1, LMAN1, PDIA4, and TXNDC5) were involved in the endoplasmic reticulum (ER) stress. Protein levels of S100A8 and S100A9 were significantly higher in RA compared to OA (for both) or to CPPA (for S100A8 only) and also significantly correlated with the histological score. Eighteen complement component proteins were identified, but only C1QB and C1QBP were weakly correlated with the histological score. This study highlights the inflammatory gradient existing between OA, CPPA and RA synovitis either at the protein level or at the histological level. Inflamed synovitis was characterized by the overexpression of ER stress proteins. © 2020, The Author(s).
Disciplines :
Rheumatology
Author, co-author :
de Seny, Dominique ;  Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Département des sciences biomédicales et précliniques
Bianchi, Elettra ;  Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Département des sciences biomédicales et précliniques
Baiwir, Dominique  ;  Université de Liège - ULiège > GIGA Platforms
COBRAIVILLE, Gaël ;  Centre Hospitalier Universitaire de Liège - CHU > Département de médecine interne > Service de rhumatologie
Collin, Charlotte ;  Centre Hospitalier Universitaire de Liège - CHU > Département de médecine interne > Service de gastroentérologie, hépatologie, onco. digestive
Deliège, Mégane ;  Centre Hospitalier Universitaire de Liège - CHU > Département de médecine interne > Service de gastroentérologie, hépatologie, onco. digestive
Kaiser, Marie-Joëlle ;  Université de Liège - ULiège > Département des sciences cliniques > Département des sciences cliniques
Mazzucchelli, Gabriel  ;  Université de Liège - ULiège > Département de chimie (sciences) > Laboratoire de spectrométrie de masse (L.S.M.)
Hauzeur, Jean-Philippe ;  Laboratory of Rheumatology, GIGA Research, CHU Liege, Tour GIGA, +2, Liege, 4000, Belgium
Delvenne, Philippe ;  Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Anatomie et cytologie pathologiques
Malaise, Michel ;  Université de Liège - ULiège > Département des sciences cliniques > Rhumatologie
Language :
English
Title :
Proteins involved in the endoplasmic reticulum stress are modulated in synovitis of osteoarthritis, chronic pyrophosphate arthropathy and rheumatoid arthritis, and correlate with the histological inflammatory score
Publication date :
2020
Journal title :
Scientific Reports
eISSN :
2045-2322
Publisher :
Nature Research
Volume :
10
Issue :
1
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 18 October 2021

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