Poster (Scientific congresses and symposiums)
Development and validation of an LC-UV method for the simultaneous analyses of ciprofloxacin and metronidazole in tablet dosage forms: routine application and confirmation of falsifications
Waffo Tchounga, Christelle Ange; Sacre, Pierre-Yves; Ciza Hamuli, Patient et al.
2021Congrès de Sciences Séparatives
 

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Keywords :
HPLC; Raman spectroscopy; medicines
Abstract :
[en] Poor quality medicines pose a threat to all health systems. It is obvious that they have harmful consequences not only from the public health point of view, but also from the economic and socio-economic as well [1]. In order to effectively combat this scourge, it is important to determine the quality of these medicines circulating on the pharmaceutical market. In general, this quality control is possible thanks to the methods listed in the pharmacopoeias. However, these methods are generally costly, not environmental friendly and often not easy to implement [2]. In this context and to overcome these disadvantages we undertook the development and validation of a rapid, less polluting and easy to implement HPLC/DAD method for the simultaneous analyses of two anti-infective very used and targeted by falsifiers, ciprofloxacin and metronidazole. Retention times of 1.7min and 3.4min were obtained for metronidazole and ciprofloxacin respectively, using a mobile phase consisting of 0.025M orthophosphoric acid adjusted to pH3 with Triethylamin and Methanol (75:25) (v/v), an XBridge column C18 (2.1*100mm) 3.5µm and a flow rate of 0.3mL/min. At first, this method was successfully validated to confirm its suitability following the total error approach with the accuracy profile as a decision tool in the laboratory of analytical pharmaceutical chemistry (LCAP) of the University of Liège and then transferred to the Laboratoire National de Contrôle des Médicaments et Expertise (LANACOME) in Cameroon to implement its usability. The acceptation limits were set at +/- 10% in total relative error and the tolerance intervals were built with a risk set at 5%. The routine application of this method allowed the identification and quantification of ciprofloxacin and metronidazole in 96 samples collected in the framework of a prevalence study in Cameroon. On the other hand, it was used as a confirmatory method to identify and quantify metronidazole that has been previously detected in tablets by handheld vibrational spectroscopy methods in 4 falsified chloroquine samples seized on the Cameroonian market during the Covid-19 pandemic.
Research center :
CIRM - Centre Interdisciplinaire de Recherche sur le Médicament - ULiège
Disciplines :
Pharmacy, pharmacology & toxicology
Author, co-author :
Waffo Tchounga, Christelle Ange  ;  Université de Liège - ULiège > Département de pharmacie > Chimie analytique
Sacre, Pierre-Yves  ;  Université de Liège - ULiège > Département de pharmacie > Chimie analytique
Ciza Hamuli, Patient  ;  Université de Liège - ULiège > CIRM
Ziemons, Eric  ;  Université de Liège - ULiège > Département de pharmacie > Chimie analytique
Hubert, Philippe;  Université de Liège - ULiège > Pharmacie > Laboratoire de Chimie Analytique Pharmaceutique
Ngono Mballa, Rose;  Université de Yaoundé I > Sciences pharmaceutiques > Pharmacologie et plantes médicinales
Marini Djang'Eing'A, Roland ;  Université de Liège - ULiège > Département de pharmacie > Chimie analytique
Language :
English
Title :
Development and validation of an LC-UV method for the simultaneous analyses of ciprofloxacin and metronidazole in tablet dosage forms: routine application and confirmation of falsifications
Publication date :
05 October 2021
Event name :
Congrès de Sciences Séparatives
Event organizer :
Association Française de Sciences Séparatives
Event place :
Paris, France
Event date :
du 05 octobre 2021 au 07 octobre 2021
Name of the research project :
Stratégies analytiques pour la détermination de la prévalence des médicaments anti-infectieux de qualité inférieure au Cameroun
Available on ORBi :
since 03 November 2021

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