Article (Scientific journals)
Inhibition of type I collagen production by dermal fibroblasts upon contact with activated T cells: different sensitivity to inhibition between systemic sclerosis and control fibroblasts.
Chizzolini, Carlo; Rezzonico, Roger; RIBBENS, Clio et al.
1998In Arthritis and Rheumatism, 41 (11), p. 2039-47
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Keywords :
Procollagen; RNA, Messenger; Interleukin-4; Interferon-gamma; Collagen; Collagenases; Matrix Metalloproteinase 1; Aged; CD4-Positive T-Lymphocytes/cytology; CD4-Positive T-Lymphocytes/immunology; CD8-Positive T-Lymphocytes/cytology; CD8-Positive T-Lymphocytes/immunology; Cell Communication/immunology; Cell Membrane/immunology; Cell Membrane/metabolism; Cells, Cultured; Collagen/genetics; Collagen/immunology; Collagenases/genetics; Extracellular Matrix/chemistry; Extracellular Matrix/immunology; Female; Fibroblasts/cytology; Fibroblasts/metabolism; Gene Expression Regulation, Enzymologic/immunology; Humans; Interferon-gamma/pharmacology; Interleukin-4/pharmacology; Lymphocyte Activation/drug effects; Lymphocyte Activation/immunology; Male; Middle Aged; Procollagen/genetics; RNA, Messenger/metabolism; Scleroderma, Systemic/immunology; Skin/cytology; Skin/immunology; Skin/metabolism; Immunology and Allergy; Rheumatology; Immunology; Pharmacology (medical)
Abstract :
[en] OBJECTIVE: To assess the role of T lymphocyte-fibroblast contact in type I collagen production by cultured dermal fibroblasts from normal individuals and from patients with diffuse systemic sclerosis (SSc). METHODS: Cell membranes were prepared from activated CD4+ and CD8+ T cells, or type 1 T helper (Th1) clones, and added to confluent fibroblast monolayers. Type I collagen production was measured in culture supernatants, and messenger RNA (mRNA) levels of type I procollagen alpha1 (pro alpha1[I]) and matrix metalloproteinase 1 (MMP-1) were evaluated by Northern hybridization analysis. RESULTS: Dose-dependent inhibition of type I collagen production was observed with CD4+ and CD8+ T cells from both SSc patients and controls. Inhibition of type I collagen was significantly less pronounced in fibroblasts from SSc patients than in fibroblasts from controls (P < 0.02). Inhibition was not reversed by the addition of exogenous transforming growth factor beta, interleukin-4, interleukin-1 receptor antagonist, anti-tumor necrosis factor, anti-CD40, or indomethacin, whereas anti-interferon-gamma (IFNgamma) reversed Th1-mediated inhibition. This inhibitory activity was specific for type I collagen, since mRNA levels of pro alpha1(I) were decreased, whereas mRNA levels of MMP-1 were strongly increased. CONCLUSION: The production of type I collagen by skin fibroblasts is specifically down-regulated by membranes from activated T cells. The contact-dependent regulatory activity exerted by T cells on fibroblasts depends, at least in part, on the presence of membrane-associated IFNgamma. However, SSc fibroblasts are more resistant to inhibition than are fibroblasts from normal individuals.
Disciplines :
Rheumatology
Author, co-author :
Chizzolini, Carlo;  University Hospital, Geneva, Switzerland
Rezzonico, Roger;  University Hospital, Geneva, Switzerland
RIBBENS, Clio ;  Université de Liège - ULiège > Département des sciences cliniques > Rhumatologie ; University Hospital, Geneva, Switzerland
Burger, Danielle;  University Hospital, Geneva, Switzerland
Wollheim, Frank A.;  University Hospital, Lund, Sweden
Dayer, Jean-Michel;  University Hospital, Geneva, Switzerland
Language :
English
Title :
Inhibition of type I collagen production by dermal fibroblasts upon contact with activated T cells: different sensitivity to inhibition between systemic sclerosis and control fibroblasts.
Publication date :
November 1998
Journal title :
Arthritis and Rheumatism
ISSN :
0004-3591
eISSN :
1529-0131
Publisher :
John Wiley & Sons, Hoboken, Us ny
Volume :
41
Issue :
11
Pages :
2039-47
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 21 April 2022

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