Crosstalk between uterine serpin (SERPINA14) and pregnancy-associated glycoproteins at the fetal-maternal interface in pregnant dairy heifers experimentally infected with Neospora caninum.

Serrano-Pérez, B; Hansen, P J; Mur-Novales, R; García-Ispierto, I; de Sousa, N M; Beckers, Jean-François; Almería, S; López-Gatius, F

doi:10.1016/j.theriogenology.2016.03.003

Article (Scientific journals)

Crosstalk between uterine serpin (SERPINA14) and pregnancy-associated glycoproteins at the fetal-maternal interface in pregnant dairy heifers experimentally infected with Neospora caninum.

Serrano-Pérez, B; Hansen, P J; Mur-Novales, R et al.

2016 • In Theriogenology, 86 (3), p. 824 - 830

Peer Reviewed verified by ORBi

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https://hdl.handle.net/2268/298232

DOI
10.1016/j.theriogenology.2016.03.003

PubMed
27045629

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Keywords :

Bovine neosporosis; Immune modulation of gestation; Placental well-being; Antibodies, Protozoan; Pregnancy Proteins; Serpins; Animals; Antibodies, Protozoan/blood; Cattle; Cattle Diseases/metabolism; Cattle Diseases/parasitology; Coccidiosis/parasitology; Coccidiosis/veterinary; Female; Gene Expression Regulation/physiology; Maternal-Fetal Exchange/physiology; Pregnancy; Pregnancy Complications, Parasitic/veterinary; Pregnancy Proteins/genetics; Pregnancy Proteins/metabolism; Serpins/genetics; Serpins/metabolism; Uterus/metabolism; Neospora; Cattle Diseases; Coccidiosis; Gene Expression Regulation; Maternal-Fetal Exchange; Pregnancy Complications, Parasitic; Uterus; Small Animals; Food Animals; Animal Science and Zoology; Equine; Pregnancy-associated glycoproteins

Abstract :

[en] Infection with Neospora caninum is the leading cause of abortion in cattle. In cows naturally infected with N caninum, plasma concentrations of pregnancy-associated glycoproteins (PAG) 1 and 2 indicate fetal-placental well-being, whereas an excess of progesterone in the second trimester of gestation has been related to high abortion rate. The immunosuppressive action of progesterone on the uterus during gestation has been attributed in part to the uterine serpins (SERPINA14). This study examines expression patterns of the genes SERPINA14, PAG, and PAG2 at the fetal-maternal interface in dairy heifers experimentally infected with N caninum during the second trimester of pregnancy, when most abortions takes place in natural conditions. Irrespective of infection, expression of SERPINA14 was higher, and expression of PAG1 and PAG2 lower, for intercaruncular endometrium than for caruncles or cotyledons. Cotyledonary tissues showed the highest expression of both PAG genes but lowest expression of SERPINA14. The expression of SERPINA14 was significantly higher in intercaruncular endometrium of control dams than for infected animals, pointing to potential disruption of modulation of maternal immune function during infection. Dramatically reduced SERPINA14 was particularly apparent in infected dams with aborted fetuses. There was also a negative association between N caninum antibody titers with SERPINA14 and PAG expression in infected animals, further suggesting that N caninum infection downregulates the uterine immunosuppressive function of SERPINA14.

Disciplines :

Veterinary medicine & animal health

Author, co-author :

Serrano-Pérez, B; Department of Animal Production, University of Lleida, 25198 Lleida, Spain, Agrotecnio Centre, University of Lleida, Spain

Hansen, P J; Department of Animal Sciences, University of Florida, USA

Mur-Novales, R; Department of Animal Production, University of Lleida, 25198 Lleida, Spain

García-Ispierto, I; Department of Animal Production, University of Lleida, 25198 Lleida, Spain, Agrotecnio Centre, University of Lleida, Spain

de Sousa, N M; Physiology of Reproduction, Faculty of Veterinary Medicine, University of Liège, Belgium

Beckers, Jean-François ; Université de Liège - ULiège > Département des sciences fonctionnelles (DSF)

Almería, S; Centre de Recerca en Sanitat Animal (CReSA), Institut de Recerca i Tecnologia Agroalimentàries (IRTA), Universitat Autònoma de Barcelona (UAB), Barcelona, Spain, Departament de Sanitat i Anatomia Animals, Universitat Autònoma de Barcelona (UAB), Barcelona, Spain

López-Gatius, F; Transfer in Bovine Reproduction SLu, Barbastro, Spain. Electronic address: lopezgatiusf@gmail.com

Language :

English

Title :

Crosstalk between uterine serpin (SERPINA14) and pregnancy-associated glycoproteins at the fetal-maternal interface in pregnant dairy heifers experimentally infected with Neospora caninum.

Publication date :

August 2016

Journal title :

Theriogenology

ISSN :

0093-691X

eISSN :

1879-3231

Publisher :

Elsevier Inc., United States

Volume :

Issue :

Pages :

824 - 830

Peer reviewed :

Peer Reviewed verified by ORBi

Additional URL :

https://api.elsevier.com/content/article/PII:S0093691X16001205?httpAccept=text/xml

Funders :

MINECO - Gobierno de Espana. Ministerio de Economia y Competitividad [ES]
FEDER - Federación Española de Enfermedades Raras [ES]

Funding text :

This study was supported by a grant from the Spanish MINECO (AGL2012-39830-C02-01/02) and FEDER . Ramón Mur-Novales was awarded an FPI grant by the Spanish Ministry of Science and Innovation, MICINN, BES-2013-063215. The authors thank Ana Burton for editorial assistance, the farmers who provided the experimental animals and the staff of CReSA for their help with managing the animals, and Dr. L.M. Ortega-Mora (SALUVET, Universidad Complutense, Madrid, Spain), for the Neospora isolate.

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