Article (Scientific journals)
Male minipuberty involves the gonad-independent activation of preoptic nNOS neurons.
Delli, Virginia; Dehame, Julien; Franssen, Delphine et al.
2023In Free Radical Biology and Medicine, 194, p. 199-208
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Keywords :
EC 1.14.13.39 (Nitric Oxide Synthase Type I); Estrogen Receptor alpha; 81R511UV73 (Pyridinolcarbamate); Gonadotropin-Releasing Hormone; Estrogens; Female; Mice; Male; Animals; Nitric Oxide Synthase Type I/genetics/metabolism; Estrogen Receptor alpha/genetics; Pyridinolcarbamate; Gonadotropin-Releasing Hormone/analysis/metabolism; Estrogens/metabolism; Gonads/chemistry/metabolism; Neurons/metabolism; Hypothalamus/metabolism; Estrogen; Gonadotropin-releasing hormone (GnRH); Hypothalamus; Neuronal nitric oxide synthase (nNOS); minipuberty
Abstract :
[en] BACKGROUND: The maturation of the hypothalamic-pituitary-gonadal (HPG) axis is crucial for the establishment of reproductive function. In female mice, neuronal nitric oxide synthase (nNOS) activity appears to be key for the first postnatal activation of the neural network promoting the release of gonadotropin-releasing hormone (GnRH), i.e. minipuberty. However, in males, the profile of minipuberty as well as the role of nNOS-expressing neurons remain unexplored. METHODS: nNOS-deficient and wild-type mice were studied during postnatal development. The expression of androgen (AR) and estrogen receptor alpha (ERα) as well as nNOS phosphorylation were evaluated by immunohistochemistry in nNOS neurons in the median preoptic nucleus (MePO), where most GnRH neuronal cell bodies reside, and the hormonal profile of nNOS-deficient male mice was assessed using previously established radioimmunoassay and ELISA methods. Gonadectomy and pharmacological manipulation of ERα were used to elucidate the mechanism of minipubertal nNOS activation and the maturation of the HPG axis. RESULTS: In male mice, minipubertal FSH release occurred at P23, preceding the LH surge at P30, when balanopreputial separation occurs. Progesterone and testosterone remained low during minipuberty, increasing around puberty, whereas estrogen levels were high throughout postnatal development. nNOS neurons showed a sharp increase in Ser(1412) phosphorylation of nNOS at P23, a phenomenon that occurred even in the absence of the gonads. In male mice, nNOS neurons did not appear to express AR, but abundantly expressed ERα throughout postnatal development. Selective pharmacological blockade of ERα during the infantile period blunted Ser(1412) phosphorylation of nNOS at P23. CONCLUSIONS: Our results show that the timing of minipuberty differs in male mice when compared to females, but as in the latter, nNOS activity in the preoptic region plays a role in this process. Additionally, akin to male non-human primates, the profile of minipuberty in male mice is shaped by sex-independent mechanisms, and possibly involves extragonadal estrogen sources.
Disciplines :
Endocrinology, metabolism & nutrition
Pediatrics
Author, co-author :
Delli, Virginia;  Univ. Lille, CHU Lille, Inserm, Laboratory of Development and Plasticity of the
Dehame, Julien;  Univ. Lille, CHU Lille, Inserm, Laboratory of Development and Plasticity of the
Franssen, Delphine ;  Université de Liège - ULiège > Département des sciences cliniques > Pédiatrie
Rasika, S;  Univ. Lille, CHU Lille, Inserm, Laboratory of Development and Plasticity of the
Parent, Anne-Simone ;  Centre Hospitalier Universitaire de Liège - CHU > > Service de pédiatrie
Prevot, Vincent;  Univ. Lille, CHU Lille, Inserm, Laboratory of Development and Plasticity of the
Chachlaki, Konstantina;  Univ. Lille, CHU Lille, Inserm, Laboratory of Development and Plasticity of the
Language :
English
Title :
Male minipuberty involves the gonad-independent activation of preoptic nNOS neurons.
Publication date :
January 2023
Journal title :
Free Radical Biology and Medicine
ISSN :
0891-5849
eISSN :
1873-4596
Publisher :
Elsevier, Amsterdam, Nl
Volume :
194
Pages :
199-208
Peer reviewed :
Peer Reviewed verified by ORBi
Commentary :
Copyright © 2022 Elsevier Inc. All rights reserved.
Available on ORBi :
since 17 January 2023

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