Article (Scientific journals)
Hyperhydricity of Prunus avium shoots cultured on gelrite: a controlled stress response
Franck, Thierry; Kevers, Claire; Gaspar, Thomas et al.
2004In Plant Physiology and Biochemistry, 42 (6), p. 519-527
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Keywords :
autophagy; cell integrity; free fatty acids; glutathione peroxidase; hyperhydricity Prunus avium; stress markers
Abstract :
[en] Hyperhydricity is a physiological disorder frequently affecting shoots vegetatively propagated in vitro. Hyperhydric shoots are characterised by a translucent aspect due to a chlorophyll deficiency, a not very developed cell wall and a high water content. Hyperhydricity of Prunus avium shoots was expressed in vitro in one multiplication cycle by replacing the gelling agent agar (normal shoots: NS) by gelrite (hyperhydric shoots: HS). P. avium shoots evolving towards the hyperhydric state produced higher amounts of ethylene, polyamines (PAs) and proline, which are substances considered as stress markers. A higher activity of glutathione peroxidase (GPX; EC 1.11.1.9), involved in organic hydroperoxide elimination, suggested an increased production of these compounds in HS. The unchanged free fatty acid composition indicated no HS membrane damages compared to NS. The ploidy level of HS nuclei was not affected, but the bigger size and the lower percentage of nuclei during the S phase suggested a slowing down of the cell cycle. The results argued for a stress response of the HS, but no signs of oxidative damages of lipid membrane and nucleus were observed. The discussion points out paradoxical results in a classical analysis of stress and suggests an alternative way of defense mechanisms in HS, involving homeostatic regulation and controlled degradation processes to maintain integrity and vital functions of the cell. (C) 2004 Elsevier SAS. All rights reserved.
Disciplines :
Biotechnology
Phytobiology (plant sciences, forestry, mycology...)
Author, co-author :
Franck, Thierry  ;  Université de Liège - ULiège > Département clinique des animaux de compagnie et des équidés > Anesthésiologie gén. et pathologie chirurg. des grds animaux
Kevers, Claire ;  Université de Liège - ULiège > Département des sciences de la vie > Biologie moléculaire et biotechnologie végétales
Gaspar, Thomas ;  Botanique, radiobotanique & serres expér.
Dommes, Jacques ;  Université de Liège - ULiège > Département des sciences de la vie > Biologie moléculaire et biotechnologie végétales
Deby, Carol
Greimers, Roland ;  Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Anatomie et cytologie pathologiques
Serteyn, Didier  ;  Université de Liège - ULiège > Département clinique des animaux de compagnie et des équidés > Anesthésiologie gén. et pathologie chirurg. des grds animaux
Deby-Dupont, Ginette;  Université de Liège - ULiège > Sciences > Centre de l'Oxygène
Language :
English
Title :
Hyperhydricity of Prunus avium shoots cultured on gelrite: a controlled stress response
Publication date :
June 2004
Journal title :
Plant Physiology and Biochemistry
ISSN :
0981-9428
Publisher :
Editions Scientifiques Medicales Elsevier, Paris, France
Volume :
42
Issue :
6
Pages :
519-527
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 28 December 2008

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