Identification of aphid salivary proteins: a proteomic investigation of Myzus persicae.

Amylases/metabolism; Animals; Aphids/enzymology/metabolism; Electrophoresis, Polyacrylamide Gel; Glucose 1-Dehydrogenase/metabolism; Glucose Oxidase/metabolism; Insect Proteins/chemistry/metabolism; NADH Dehydrogenase/metabolism; Proteomics/methods; Salivary Proteins and Peptides/chemistry/metabolism; Spectrometry, Mass, Electrospray Ionization; Tandem Mass Spectrometry; Trypsin/metabolism; alpha-Glucosidases/metabolism

Abstract :

[en] The role of insect saliva in the first contact between an insect and a plant is crucial during feeding. Some elicitors, particularly in insect regurgitants, have been identified as inducing plant defence reactions. Here, we focused on the salivary proteome of the green peach aphid, Myzus persicae. Proteins were either directly in-solution digested or were separated by 2D SDS-PAGE before trypsin digestion. Resulting peptides were then identified by mass spectrometry coupled with database investigations. A homemade database was constituted of expressed sequence tags from the pea aphid Acyrtosiphon pisum and M. persicae. The databases were used to identify proteins related to M. persicae with a nonsequenced genome. This procedure enabled us to discover glucose oxidase, glucose dehydrogenase, NADH dehydrogenase, alpha-glucosidase and alpha-amylase in M. persicae saliva. The presence of these enzymes is discussed in terms of plant-aphid interactions.

Disciplines :

Biochemistry, biophysics & molecular biology

Author, co-author :

Harmel, Nicolas ; Université de Liège - ULiège > Gembloux Agro-Bio Tech > Gembloux Agro-Bio Tech

Letocart, E.

Cherqui, A.

Giordanengo, P.

Mazzucchelli, Gabriel ; Université de Liège - ULiège > Centre interfac. d'analyse des résidus en traces (CART)

Guillonneau, F.

De Pauw, Edwin ; Université de Liège - ULiège > Département de chimie (sciences) > GIGA-R : Laboratoire de spectrométrie de masse (L.S.M.)

Haubruge, Eric ; Université de Liège - ULiège > Services administratifs généraux > Vice-Recteur pour le site de Gembloux

Francis, Frédéric ; Université de Liège - ULiège > Gembloux Agro-Bio Tech > Gembloux Agro-Bio Tech

Language :

English

Title :

Identification of aphid salivary proteins: a proteomic investigation of Myzus persicae.

Publication date :

2008

Journal title :

Insect Molecular Biology

ISSN :

0962-1075

eISSN :

1365-2583

Publisher :

Blackwell Publishing, Oxford, United Kingdom

Volume :

Issue :

Pages :

165-74

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 02 January 2010

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Scopus citations^®

194

Scopus citations^®
without self-citations

178

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171

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