[en] RT-PCR was used to assay for growth factors and receptors from seven different protein families in cochlea tissues of the juvenile rat. There was a broad representation of the growth factor families in all the cochlea tissues examined, though the organ of Corti and stria vascularis expressed a greater variety than the spiral ganglion. This broad expression suggests that a variety of known growth factors play significant roles in the development, maintenance, and repair of the inner ear. The results of this survey serve as a basis for the design of future in vitro experiments that will address the ability of growth factors to protect hair cells from damage and to evoke a repair-regeneration response by injured hair cells.
Malgrange, Brigitte ; Université de Liège - ULiège > CNCM/ Centre fac. de rech. en neurobiologie cell. et moléc.
Rogister, Bernard ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Biochimie et physiologie générales, et biochimie humaine
Ernfors, P., Van De Water, T. R., Loring, J., and Jaenisch, R. 1995. Complementary roles of BDNF and NT-3 in vestibular and auditory development. Neuron. 14:1153-1164.
Staecker, H., Lefebvre, P. P., Malgrange, B., Moonen, G., and Van De Water, T. R. 1995. Regeneration and mammalian auditory hair cells. Response. Science. 267:709-711.
Chomczynski, P., and Sacchi, N. 1987. A rapid method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal. Biochem. 162:156-159.
Luo, L., Koutnouyan, H., Baird, A., and Ryan, A. F. 1993. Acidic and basic FGF mRNA expression in the adult and developing rat cochlea. Hear. Res. 69:182-193.
Pirvola, U., Cao, Y., Oellig, C., Suoqiang, Z., Pettersson, R. F., and Ylikoski, J. 1995. The site of action of neuronal acidic fibroblast growth factor is the organ of Corti of the rat cochlea. Proc. Natl. Acad. Sci, USA. 92:9269-9273.
Lee, K. H., and Cotanche, D. A. 1996. Potential role of bFGF and retinoic acid in the regeneration of chicken cohlear hair cells. Hear. Res. 94:1-13.
Pirvola, U., Arumäe, U., Moshnyakov, M., Palgi, J., Saarma, M., and Ylikoski, J. 1994. Coordinated expression and function of neurotrophins and their receptors in the rat inner ear during target innervation. Hear. Res. 75:131-144.
Ylikoski, J., Pirvola, U., Moshnyakov, M., Palgi, J., Arumäe, U., and Saarma, M. 1993. Expression patterns of neurotrophin and their receptor mRNAs in the rat inner ear. Hear. Res. 65/1-2:69-78.
Schecterson, L. C., and Bothwell, M. 1994. Neurotrophin and neurotrophin receptor mRNA expression in developing inner ear. Hear. Res. 73:92-100.
Saffer, L. D., Gu, R., and Corwin, J. T. 1996. An RT-PCR analysis of mRNA for growth factor receptors in damaged and control sensory epithelia of rat utricules. Hear. Res. 94:14-23.
Rybak, L. P., Whitworth, C., Scott, V., and Weberg, A. 1991. Ototoxicity of furosemide during development. Laryngoscope. 101:1167-1174.
Sagara, T., Furukawa, K., Makishima, K., and Fujimoto, S. 1995. Differentiation of the rat stria vascularis. Hear. Res. 83:121-132.
Staecker, H., Kopke, R., Malgrange, B., Lefebvre, P. P., and Van De Water, T. R. 1996. NT-3 and/or BDNF therapy prevents loss of auditory neurons following loss of hair cells. Neuroreport. 7: 889-894.
Ernfors, P., Duan, M. L., ElShamy, W. M., and Canlon, B. 1996. Protection of auditory neurons from aminoglycoside toxicity by neurotrophin-3. Nature Medicine. 2:463-467.
Malgrange, B., Lefebvre, P. P., Van De Water, T. R., Staecker, H., and Moonen, G. 1996. Effects of neurotrophins on early auditory neurons in cell culture. Neuroreport. 7:913-917.
Malgrange, B., Lefebvre, P. P., Martin, D., Staecker, H., Van De Water, T. R., and Moonen, G. 1996. NT-3 has a tropic effect on process outgrowth by postnatal auditory neurons in vitro. Neuroreport. 7:2495-2499.
Hartnick, C., Staecker, H., Malgrange, B., Lefebvre, P. P., Liu, W., Moonen, G., and Van De Water, T. R. 1996. Neurotrophic effects of BDNF and CNTF, alone and in combination, on postnatal day 5 rat acoustic ganglion neurons. J. Neurobiol. 30:246-254.
Schimmang, T., Minichiello, L., Vazquez, E., San Jose, I., Giraldez, F., Klein, R., and Represa, J. 1995. Developing inner ear sensory neurons require trkB and trkC receptors for innervation of their peripheral targets. Development. 121:3381-3391.
Wetmore, C., and Olson, L. 1995. Neuronal and Nonneuronal expression of neurotrophins and their receptors in sensory and sympathetic ganglia suggest new intercellular trophic interactions. J. Comp. Neurol. 353:143-159.
