[en] The structural arrangement of type I collagen in vivo is critical for the normal functioning of tissues, such as bone,
cornea, and blood vessels. At present, there are no low-cost techniques for fabricating aligned collagen structures for
applications in regenerative medicine. Here, we report a straightforward approach to fabricate collagen films, with
defined orientation of collagen fibrillar aggregates within a matrix of oriented collagen molecules. Langmuir-Blodgett
(LB) technology was used to deposit thin films of oriented type I collagen onto substrates. It was found that collagen
does not behave like classical LB materials, such as amphiphilic hydrocarbon acids or lipids. The thickness of the
deposited collagen films and the area-pressure isotherms were found to depend on the amount of material spread. In
addition, no film collapse was detected and the deposited LB films were thicker than the theoretical dimension of a
collagen monolayer (1.5 nm) formed by triple helical collagen molecules. Individual LB films with thicknesses of up to
20 nm were obtained, and multiple depositions yielded overall film thicknesses of up to 100 nm. Films consisted of a
matrix of collagen molecules containing thicker fibrillar aggregates of collagen (micrometers in length). These fibrillar
aggregates were built up of shorter unit molecules forming “spun thread” structures, some of which exhibited a zigzag
pattern. In addition to aligning collagen unidirectionally (similar for example to tendon), we performed a two-step
deposition procedure, in which the substrate was turned 90 between two consecutive collagen deposition steps. The
resulting films showed orthogonally aligned collagen layers, mimicking the structure of cornea. Thus, this technique
permits control of the thickness of individual layers, the orientation of successive layers, and the number of layers within
the construct. Therefore, it may have widespread applicability for the engineering of collagen-rich tissues
Disciplines :
Chemistry
Author, co-author :
Tenboll, Annabell; The University of Western Ontario, London, Canada
Behafarid, Darvish; The University of Western Ontario, London, Canada
Hou, Weimin; University of Liege > Department of Chemistry
Duwez, Anne-Sophie ; Université de Liège - ULiège > Département de chimie (sciences) > Nano-chimie et systèmes moléculaires
Dixon, S. Jeffrey; The University of Western Ontario, London, Canada
Goldberg, Harvey A.; The University of Western Ontario, London, Canada
Grohe, Bernd; The University of Western Ontario, London, Canada
Mittler, Silvia; The University of Western Ontario, London, Canada
Language :
English
Title :
Controlled Deposition of Highly Oriented Type I Collagen Mimicking In Vivo Collagen Structures
Publication date :
2010
Journal title :
Langmuir
ISSN :
0743-7463
eISSN :
1520-5827
Publisher :
American Chemical Society, Washington, United States - District of Columbia
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