Assessment of plasma profile of pregnancy-associated glycoprotein (PAG) in sheep with a heterologous (anti-caPAG(55+59)) RIA diagnosing and its potential for pregnancy
Ledezma-Torres, R. A.; Beckers, Jean-François; Holtz, W.
[en] The purpose of the present investigation was to generate pregnancy associated glycoprotein (PAG)-profiles throughout pregnancy in a heterogenous sample of sheep using a radioimmunoassay with a heterologous antibody (anti-caPAG55,59, #708) and utilize them for the purpose of pregnancy detection. From 2 weeks after the introduction of males into the breeding herd until 4 weeks after parturition, weekly blood samples were collected from 66 pregnant and 25 non-pregnant ewes of various breeds. Between 3 and 5 weeks after conception, plasma PAG levels increased, remained almost stable until week 17, then continued to increase, culminating in a drastic surge during the last 2 weeks of pregnancy. By 4 weeks of gestation, the plasma PAG level exceeded the level typical for non-pregnant ewes by five standard deviations, permitting a reliable pregnancy diagnosis. Plasma PAG levels were higher in twin-bearing ewes than in ewes carrying a single lamb, differences getting more evident as pregnancy proceeded. Neither breed and parity of the mother nor sex and weight of lambs home exerted a significant effect. The heterologous assay system utilizing a caprine antibody proved to deliver results that are more consistent and less depending on various variables than those used in other studies. It may be concluded that, at the present state of development, the assay provides a reliable means of diagnosing pregnancy in sheep from the 4th week after they have been bred onward. (c) 2006 Elsevier Inc. All rights reserved.
Disciplines :
Veterinary medicine & animal health
Author, co-author :
Ledezma-Torres, R. A.
Beckers, Jean-François ; Université de Liège - ULiège > Département de sciences fonctionnelles > Physiologie de la reproduction
Holtz, W.
Language :
English
Title :
Assessment of plasma profile of pregnancy-associated glycoprotein (PAG) in sheep with a heterologous (anti-caPAG(55+59)) RIA diagnosing and its potential for pregnancy
Cole H.H., and Hart G.H. The potency of blood serum of mares in progressive stages of pregnancy in effecting the sexual maturity of the immature rat. Am J Physiol 93 (1930) 57-68
Robertson H.A., Chan J.S.D., Hackett A.J., Marcus G.J., and Friesen H.G. Diagnosis of pregnancy in the ewe at mid-gestation. Anim Reprod Sci 3 (1980) 69-71
Zoli A.P., Beckers J.F., and Ectors F. Isolement et caractérisation partielle d'une glycoprotéine associée à la gestation chez la brebis. Ann Méd Vét 139 (1995) 177-184
Ruder C.A., Stellflug J.N., Dahmen J.J., and Sasser R.G. Detection of pregnancy in sheep by radioimmunoassay of sera for pregnancy-specific protein B. Theriogenology 29 (1988) 905-912
Butler J.E., Hamilton W.C., Sasser R.G., Ruder C.A., Hass G.M., and Williams R.J. Detection and partial characterization of two bovine pregnancy-specific proteins. Biol Reprod 26 (1982) 916-925
Zoli A.P., Beckers J.F., Wouters-Ballman P., Closset J., Falmagne P., and Ectors F. Purification and characterization of bovine pregnancy-associated glycoprotein. Biol Reprod 45 (1991) 1-10
Osborn D.A., Beckers J.F., Sulon J., Gassett J.W., Muller L.I., Murphy B.P., et al. Use of glycoprotein assays of pregnancy diagnosis in white-tailed deer. J Wild Manage 60 (1996) 388-393
Garbayo J.M., Remy B., Alabart J.L., Folch J., Wattiez R., Falmagne P., et al. Isolation and partial characterization of a pregnancy-associated glycoprotein family from the goat placenta. Biol Reprod 58 (1998) 109-115
Xie S., Green J.A., Bao B., Beckers J.F., Valdez K.E., Hakami L., et al. Multiple pregnancy-associated glycoproteins are secreted by day 100 ovine placental tissue. Biol Reprod 57 (1997) 1384-1393
Green J.A., Xie S., Quan X., Bao B., Gan X., Mathialagan N., et al. Pregnancy-associated bovine and ovine glycoproteins exhibit spatially and temporally distinct expression patterns during pregnancy. Biol Reprod 62 (2000) 1624-1631
Garbayo J.M., Green J.A., Manikkam M., Beckers J.F., Kiesling D.O., Ealy A.D., et al. Caprine pregnancy-associated glycoproteins (PAG): their cloning, expression, and evolutionary relationship to other PAG. Mol Reprod Dev 57 (2000) 311-322
Roberts R.M., Xie S., and Mathialagan N. Maternal recognition of pregnancy. Biol Reprod 54 (1996) 294-302
Weems CW, Weems YS, Randel RD. Prostaglandins and reproduction in farm animals. Vet J [in print, doi:10.1016/j.tvjl.2004.11.014].
El Amiri B. Investigations on pregnancy-associated glycoproteins (PAG's) in sheep: isolation, purification and characterization (2003), Presses de la Faculté de Médicine Véterinaire de l'Univerité de Liège, B-4000 Liège Belgique ISBN 2-930212-76-4
Zoli A.P., Guilbault L.A., Delahaut P., Benítez Ortiz W., and Beckers J.F. Radioimmunoassay of a bovine pregnancy-associated glycoprotein in serum: Ist application for pregnancy diagnosis. Biol Reprod 46 (1992) 83-92
Szenci O., Beckers J.F., Humblot P., Sulon J., Sasser G., Taverne M.A.M., et al. Comparison of ultrasonography, bovine pregnancy-specific protein B, and bovine pregnancy-associated glycoprotein 1 tests for pregnancy detection in dairy cows. Theriogenology 50 (1998) 77-88
González F., Sulon J., Garbayo J.M., Batista M., Cabrera F., Calero P.O., et al. Early pregnancy diagnosis in goats by determination of pregnancy-associated glycoprotein concentrations in plasma samples. Theriogenology 52 (1999) 717-725
Zarrouk A., Engeland I., Sulon J., and Beckers J.F. Determination of pregnancy-associated glycoprotein concentrations in goats (Capra hircus) with unsuccessful pregnancies: a retrospective study. Theriogenology 51 (1999) 1321-1331
Rodbard D. Statistical and quality control and routine data processing for radioimmunoassays and immunoradiometric assays. Clin Chem 10 (1974) 1255-1270
Skelley D.S., Brown L.P., and Besch P.K. Radioimmunoassay. Clin Chem 19 (1973) 146-186
Statistical Analysis System Institute. SAS/STAT User's Guide, Release 6.08 TS407 ed., SAS Institute, Cary, NC, USA, 1989.
Ranilla M.J., Sulon J., Carro M.D., Mantecón A.R., and Beckers J.F. Plasmatic profiles of pregnancy-associated glycoprotein and progesterone levels during gestation in churra and merino sheep. Theriogenology 42 (1994) 537-545
Ranilla M.J., Sulon J., Mantecon A.R., Beckers J.F., and Carro M.D. Plasma pregnancy-associated glycoprotein and progesterone concentrations in pregnant Assaf ewes carrying single and twin lambs. Small Rum Res 24 (1997) 125-131
Gajewski Z., Beckers J.F., Melo de Sousa N., Thun R., Sulon J., and Faundez R. Oznaczanie stezenia PAG u owiec badania retrospektywne. Adv Cell Biol 26 (1999) 89-96
Sousa N.M., Garbayo J.M., Figueiredo J.R., Sulon J., Gonccalves P.B.D., and Beckers J.F. Pregnancy-associated glycoprotein and progesterone profiles during pregnancy and postpartum in native goats from the north-east of Brazil. Small Rum Res 1739 (1999) 1-11
Fernández-Arias A., Alabart J.L., Folch J., and Beckers J.F. Interspecies pregnancy of Spanish ibex (Capra pyrenaica) fetus in domestic goat (Capra hircus) recipients induces abnormally high plasmatic levels of pregnancy-associated glycoprotein. Theriogenology 51 (1999) 1419-1430
González F., Sulon J., Garbayo J.M., Batista M., Cabrera F., Calero P.O., et al. Secretory profiles of pregnancy-associated glycoproteins at different stages of pregnancy in the goat. Reprod Dom Anim 35 (2000) 79-82
Karen A., Beckers J.F., Sulon J., Sousa N.M., Szabados K., Reczigel J., et al. Early pregnancy diagnosis in sheep by progesterone and pregnancy-associated glycoprotein test. Theriogenology 59 (2003) 1941-1948
El Amiri B., Remy B., Sousa N.M., Gerardin-Otthiers N., Desbuleux H., Perenyi Z., et al. Purification and characterization of a pregnancy-associated glycoprotein (ovPAG-6) from sheep placenta removed between 66 and 100 days of gestation. Biotechnol Agron Soc Environ 6 (2002) 11-12 [abstr.]
Patel O.V., Sulon J., Beckers J.F., Takahashi T., Hirako M., Sasaki N., et al. Plasma bovine pregnancy-associated glycoprotein concentrations throughout gestation in relationship to fetal number in the cow. Eur J Endocrinol 137 (1997) 423-428
