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Possible Enhancing Mechanisms for Gene Transfer Activity of Glucuronylglucosyl-β-cyclodextrin/dendrimer Conjugate
http://hdl.handle.net/2298/26422
http://hdl.handle.net/2298/264220dcc2894-2219-4584-bc12-58f2d2d6fc10
| 名前 / ファイル | ライセンス | アクション |
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Fig-IJP-GUG-R1-submit.pdf (324.8 kB)
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IJP-Anno-arima-Kumamoto University-Archive-2.pdf (352.5 kB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||
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| 公開日 | 2013-01-17 | |||||
| タイトル | ||||||
| タイトル | Possible Enhancing Mechanisms for Gene Transfer Activity of Glucuronylglucosyl-β-cyclodextrin/dendrimer Conjugate | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 主題 | PAMAM starburst dendrimer, glucuronylglucosyl-β-cyclodextrin, conjugate, cyclodextrins, non-viral vector, enhancing mechanism | |||||
| 資源タイプ | ||||||
| 資源タイプ | journal article | |||||
| 著者 |
Anno, Takayuki
× Anno, Takayuki× Higashi, Taishi× Motoyama, Keiichi× Hirayama, Fumitoshi× Uekama, Kaneto× Arima, Hidetoshi |
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| 別言語の著者 |
安野, 貴幸
× 安野, 貴幸× 東, 大志× 本山, 敬一× 平山, 文俊× 上釜, 兼人× 有馬, 英俊 |
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| 内容記述 | ||||||
| 内容記述 | We previously reported that glucuronylglucosyl-β-cyclodextrin (GUG-β-CyD) conjugate with polyamidoamine starburst dendrimer (GUG-β-CDE conjugate) with the average degree of substitution (DS) of cyclodextrin (CyD) of 1.8 (GUG-β-CDE conjugate (DS 1.8)), showed remarkably higher gene transfer activity than α-cyclodextrin/dendrimer conjugate (α-CDE conjugate (DS 1.2)) and β-cyclodextrin/dendrimer conjugate (β-CDE conjugate (DS 1.3)) in vitro and in vivo. In this study, to clarify the enhancing mechanism for high gene transfer activity of GUG-β-CDE conjugate (DS 1.8), we investigated the physicochemical properties, cellular uptake, endosomal escape and nuclear translocation of the plasmid DNA (pDNA) complexes as well as pDNA release from the complexes. The particle size, ς-potential and cellular uptake of GUG-β-CDE conjugate (DS 1.8)/pDNA complex were mostly comparable to those of α-CDE conjugate (DS 1.2) and β-CDE conjugate (DS 1.3). Meanwhile, GUG-β-CDE conjugate (DS 1.8)/pDNA complex was likely to have high endosomal escaping ability and nuclear localization ability in A549 and RAW264.7 cells. In addition, the pDNA condensation and decondensation abilities of GUG-β-CDE conjugate (DS 1.8) were lower and higher than that of α-CDE conjugate (DS 1.2) or β-CDE conjugate (DS 1.3), respectively. These results suggest that high gene transfer activity of GUG-β-CDE conjugate (DS 1.8) could be, at least in part, attributed to high endosomal escaping ability, nuclear localization ability and suitable pDNA release of its complex. | |||||
| 書誌情報 |
International Journal of Pharmaceutics 巻 426, 号 1-2, p. 239-247, 発行年 2012-04-15 |
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| ISSN | ||||||
| 収録物識別子 | 03785173 | |||||
| PubMed番号 | ||||||
| 関連タイプ | isVersionOf | |||||
| 識別子タイプ | PMID | |||||
| 関連識別子 | 22306427 | |||||
| DOI | ||||||
| 関連タイプ | isVersionOf | |||||
| 関連識別子 | 10.1016/j.ijpharm.2012.01.039 | |||||
| 権利 | ||||||
| 権利情報 | © 2012 Elsevier | |||||
| フォーマット | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | application/pdf | |||||
| フォーマット | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | application/pdf | |||||
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| 352502 bytes | ||||||
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| 324824 bytes | ||||||
| 著者版フラグ | ||||||
| 出版タイプ | AM | |||||
| 日本十進分類法 | ||||||
| 主題Scheme | NDC | |||||
| 主題 | 499 | |||||
| 出版者 | ||||||
| 出版者 | Elsevier | |||||
| 資源タイプ | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | 論文(Article) | |||||
| 資源タイプ・ローカル | ||||||
| 雑誌掲載論文 | ||||||
| 資源タイプ・NII | ||||||
| Journal Article | ||||||
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| URL | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | http://www.sciencedirect.com/science/article/pii/S0378517312000932 | |||||
