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＜departmental bulletin paper＞
Injuring Reaction of δ-Endotoxin upon Sarcoma 180 Ascites Cells and Silkworm Midgut Cells in vitro

Creator
Creator Name
関, 武純
Seki, Takeyoshi
Affiliation
Affiliation Name
九州大学農学部蚕糸化学教室
Laboratory of Sericultural Chemistry, Faculty of Agriculture, Kyushu University
Creator Name
永松, 幹晴
Nagamatsu, Mikiharu
Affiliation
Affiliation Name
九州大学農学部蚕糸化学教室
Laboratory of Sericultural Chemistry, Faculty of Agriculture, Kyushu University
Creator Name
永松, 康徳
Nagamatsu, Yasunori
Affiliation
Affiliation Name
九州大学農学部蚕糸化学教室
Laboratory of Sericultural Chemistry, Faculty of Agriculture, Kyushu University
Creator Name
筒井, 亮毅
Tsutsui, Ryoki
Affiliation
Affiliation Name
九州大学農学部蚕糸化学教室
Laboratory of Sericultural Chemistry, Faculty of Agriculture, Kyushu University
Creator Name
市丸, 哲造
Ichimaru, Tetsuzo
Affiliation
Affiliation Name
九州大学農学部蚕糸化学教室
Laboratory of Sericultural Chemistry, Faculty of Agriculture, Kyushu University
Author PID
L000569
Creator Name
渡辺, 忠雄
Watanabe, Tadao
Affiliation
Affiliation Name
九州大学農学部蚕糸化学教室
Laboratory of Sericultural Chemistry, Faculty of Agriculture, Kyushu University
Creator Name
古賀, 克己
Koga, Katsumi
Affiliation
Affiliation Name
九州大学農学部蚕糸化学教室
Laboratory of Sericultural Chemistry, Faculty of Agriculture, Kyushu University
Creator Name
林, 勝哉
Hayashi, Katsuya
Affiliation
Affiliation Name
九州大学農学部蚕糸化学教室
Laboratory of Sericultural Chemistry, Faculty of Agriculture, Kyushu University
Language
Japanese
Publisher
九州大學農學部
Faculty of Agriculture, Kyushu University
Date
1978-09
Source Title
Science bulletin of the Faculty of Agriculture, Kyushu University
Vol
33
Issue
1
First Page
19
Last Page
24
Publication Type
Version of Record
Access Rights
open access
JaLC DOI
https://doi.org/10.15017/23264
Related DOI
九州大學農學部學藝雜誌 || 33(1) || p19-24
Science bulletin of the Faculty of Agriculture, Kyushu University || 33(1) || p19-24
http://www.agr.kyushu-u.ac.jp/
Related URI
九州大學農學部學藝雜誌 || 33(1) || p19-24
Science bulletin of the Faculty of Agriculture, Kyushu University || 33(1) || p19-24
http://www.agr.kyushu-u.ac.jp/
Relation
九州大學農學部學藝雜誌 || 33(1) || p19-24
Science bulletin of the Faculty of Agriculture, Kyushu University || 33(1) || p19-24
http://www.agr.kyushu-u.ac.jp/

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Details

Record ID
23264
Peer-Reviewed
Unrefereed
ISSN
0368-6264
NCID
AN0005519X
Notes
The insecticidal 8-endotoxin produced by Bacillus thuringiensis strain T 84 A1 was found to attack mouse sarcoma 180 ascites cells in vitro, stimulating the release of UV-absorbing substances from the cells into the medium. For this effect it was required that the proteinaceous endotoxin was previously solubilized by gut-juice protease from the silkworm or by a reducing agent. The substances released had a UV-spectrum like that of RNA. Phospholipid was also liberated significantly from sarcoma cells in the presence of solubilized endotoxin. After the midgut of the silkworm, which may be the primary target organ of the toxin, was suspended in a medium containing solubilized toxin, it released significant amounts of UV-absorbing substances into the medium. Similar effects were observed for free cells prepared from the silkworm midgut treated with trypsin. In the latter experiments, however, nonspecific liberation of UV-absorbancy was often observed even in the absence of toxin, suggesting that the intactness of free cells after trypsin-treatment may be critical.
Bacillus thuringiensis T84 A1 株の産生するδ-内毒素は,カイコ消化液プロテアーゼなどによりあらかじめ可溶化しておくと,マウスザルコーマ180腹水ガン細胞にin vitroで作用して,培地への紫外吸収物質の漏出を著しく促進することがわかつた.漏出された物質は,260nmに極大吸収をもつRNA様のものであつた.また,可溶化毒素はザルコーマ細胞からのリン脂質の遊離をも促進した.この毒素の実際の標的器官であるといわれる中腸組織(カイコ)を,可溶化した毒素を含む培地中に浸しておくと,培地に紫外吸収物質の放出が観察された.カイコ中腸組織からトリプシン処理により遊離の細胞をとつて毒素で処理しても同様の結果が得られた.ただしカイコの遊離細胞の実験では,毒素に対して特異な反応を示すような無傷の細胞を得ることが必要で的あつた.
Created Date 2012.07.25
Modified Date 2022.11.11

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